1. Genetic Monitoring of Sockeye Reintroduction in Cle Elum Lake
Timothy Knepp
Andrew P. Matala, Shawn R. Narum, Peter F. Galbreath
Columbia River Inter-Tribal Fish Commission
Brian Saluskin, Mark Johnston
The Confederated Tribes and Bands of the Yakama Nation

2. The 2014 return

3. life histories comparison between source stocks Wenatchee Osoyoos 4-5
Sept.-Oct.
cool headwater
stream spawners
3-4
Oct.-Nov.
Eutrophic
shore spawners
spawner age
spawn time
habitat
Lake Osoyoos has a higher production of planktonic biomass. Details of the procedure used as the basis to differentiate smolt stock of origin (Fryer 1994, 1995).
rearing
size
outmigration
1 year
FL ~83mm
Earlier (?)
1 year
FL >100mm
Later (?)

4. genetically distinct stocks
Wenatchee
Osoyoos
Lake Osoyoos has a higher production of planktonic biomass. Details of the procedure used as the basis to differentiate smolt stock of origin (Fryer 1994, 1995).

5. yes
Genetic stock ID:
Its like Magic
Cle Elum unknown

6. What has been outplanted?
4,500
10,000
4,000
10,000
10,000
Osoyoos
% sample
Wenatchee
75%
84%
92%
66%
60%

7. Other interesting stuff and things
Wenatchee donor
Osoyoos donor
Redfish Lake
sockeye
Keechelus
Naches
Lake Whatcom
kokanee (n)
outplants
n=2 (Redfish)
n=1

8. Stock distinctions maintained In Cle Elum Lake, or reservoir?
……..whatever

9. Stock distinctions maintained In Cle Elum Lake, or reservoir?
……..whatever
Stocks remain reproductively isolated

10. 2015 survey by reach
Osoyoos
Wenatchee

11. Temporal more than spatial
survey by section
Osoyoos
Wenatchee
Temporal more than spatial

12. shore spawning Osoyoos sockeye bycatch during lake trout gill netting
outplant
release
19% total
Sections 1&2
Osoyoos sockeye bycatch
during lake trout gill netting

13. What is outmigrating?
85%
50%
39%
56%
Osoyoos
% sample
Wenatchee

14. Other interesting stuff and things
Wenatchee donor
Osoyoos donor
Redfish Lake
sockeye
Keechelus
Naches
Lake Whatcom
kokanee (n)
juveniles
n=63

15. Juvenile statistics: outmigration time & size
fork length (mm)
sample proportion

16. Juvenile statistics: outmigration time & size
ordinal day
sample proportion
fork length (mm)

17. What is returning? Osoyoos Wenatchee % sample
The “first” wild return (2013)
148
688
2,541 93
Osoyoos
% sample
Wenatchee
98%
65%
46%
82%

18. Return statistics: size distribution
507
559
sample proportion
402
499
559
sample proportion
fork length (mm)

19. Return statistics: size distribution

20. Replacement?......Maybe
2009 outplants = 1000
2010 outplants = 2600

21. Replacement?......Maybe
2009 outplants = 1000
2010 outplants = 2600
~1/2 of these may be 3 year olds

22. Return migration time Zosel Dam counts: Osoyoos stock
high temps = delayed migration
August 11th
% total return
ordinal day

23. Return migration time Cle Elum Returns: Osoyoos stock
high temps = delayed migration
August 9th
% total return
ordinal day

24. Return migration time Tumwater Dam counts: Wenatchee stock
Cool temps = rapid migration
July 16th
% total return
ordinal day

25. Return migration time Cle Elum Returns: Wenatchee stock
high temps = delayed migration
August 9th
% total return
ordinal day

26. Return migration time
2015
% total return
ordinal day

27. Are life stage proportions indicative of
acclimation potential – tolerance?
outplants
n=2 (Redfish)
juveniles
n=63
returns
n=2

28. What to focus on in coming years..
Two stock approach
Life history distinctions are a bet hedge; maximize
habitat
Age data for returns and smolts will be critical
Temporal differences in adult size-at-age may be
indicative of smolt age at outmigration
Parentage to commence shortly following
marker development
First F2s will return this year; age 3 returns
from wild production in 2013

29. Questions?