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West Nile virus outbreak in Israel in 2015: phylogenetic and geographic characterization in humans and mosquitoes  Y. Lustig, Z. Kaufman, B. Mannasse,

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Presentation on theme: "West Nile virus outbreak in Israel in 2015: phylogenetic and geographic characterization in humans and mosquitoes  Y. Lustig, Z. Kaufman, B. Mannasse,"— Presentation transcript:

1 West Nile virus outbreak in Israel in 2015: phylogenetic and geographic characterization in humans and mosquitoes  Y. Lustig, Z. Kaufman, B. Mannasse, R. Koren, S. Katz-Likvornik, L. Orshan, A. Glatman-Freedman, E. Mendelson  Clinical Microbiology and Infection  Volume 23, Issue 12, Pages (December 2017) DOI: /j.cmi Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

2 Fig. 1 Prevalence of West Nile virus (WNV) human infections and mosquito pools in Israel. Residence (by address) and trapping sites, locations of acute WNV patients and WNV-infected mosquito pools, respectively, were overlaid on the geographical map of Israel and number of human cases per 100 000 inhabitants was calculated. Different colours show the prevalence range in each area and black dot, the WNV-positive mosquito pools. Names of areas with high prevalence of acute infection or with WNV-positive mosquito pools are indicated. Clinical Microbiology and Infection  , DOI: ( /j.cmi ) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

3 Fig. 2 Phylogenetic tree of lineage 1 West Nile viruses (WNVs) from Israel, The analysis was conducted on a nucleotide sequence of the genes encoding the capsid, envelope, pre-membrane and membrane proteinS (1385 nucleotides), using the neighbour-joining method implemented in MEGA 6.0 software. The robustness of branching pattern was tested by 1000 bootstrap replications. The percentage of successful bootstrap replicates is indicated at nodes, showing only values of >70%. The bar denotes 0.02 nucleotide substitutions per site. WNV lineage 1 strains sequenced in this study are shown in black diamonds (human isolations) and open circles (mosquito isolations). The number followed by an ‘m’ or ‘H’ indicates mosquito pool or human isolate, respectively, and the two digits at the end indicate the year the pool was collected. Lineage 1 reference strains are underlined, and the country and year of isolation are presented in parenthesis. A sequence obtained from strain MRM 16 (GenBank accession no. GQ851602) was used as an out-group. Clinical Microbiology and Infection  , DOI: ( /j.cmi ) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

4 Fig. 3 Amino acid substitutions in West Nile virus (WNV) strains isolated from humans and mosquitoes. The 461-amino-acid sequence of 27 WNV isolates from humans (ten WNV Mediterranean subtype strains) and mosquitoes (six WNV Mediterranean and 11 WNV Eastern European subtypes strains) were aligned and compared with WNV Mediterranean strain wnv_398m_ISR_15. All 28 WNV strains were isolated in 2015 in Israel. Mutated residues (compared with wnv_398m_ISR_15) are indicated in grey as well as their position in the WNV polypeptide. Clinical Microbiology and Infection  , DOI: ( /j.cmi ) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

5 Fig. 4 Spatial distribution of West Nile virus (WNV) strains sequenced from human patients and mosquito pools. Residence (by address) and trapping sites locations of sequenced acute WNV patients and sequenced WNV-infected mosquito pools, respectively, are shown. Human cases (all with WNV Mediterranean subtype) are depicted by red triangles. Cyan and violet circles represent mosquitoes infected with WNV Eastern European (E) or Mediterranean (M) subtypes, respectively. Details on each isolate are specified in Tables 1 and 2 according to the numbers indicated on the map. Clinical Microbiology and Infection  , DOI: ( /j.cmi ) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions


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