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Radiation Sources Providing Increased UVA/UVB Ratios Attenuate the Apoptotic Effects of the UVB Waveband UVA-Dose-Dependently in Hairless Mouse Skin 

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Presentation on theme: "Radiation Sources Providing Increased UVA/UVB Ratios Attenuate the Apoptotic Effects of the UVB Waveband UVA-Dose-Dependently in Hairless Mouse Skin "— Presentation transcript:

1 Radiation Sources Providing Increased UVA/UVB Ratios Attenuate the Apoptotic Effects of the UVB Waveband UVA-Dose-Dependently in Hairless Mouse Skin  Yuko Ibuki, Munif Allanson, Katie M. Dixon, Vivienne E. Reeve  Journal of Investigative Dermatology  Volume 127, Issue 9, Pages (September 2007) DOI: /sj.jid Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Formation of sunburn cells after exposure to SSUV. (a) Light microscopy of histochemical identification (H&E stain) of sunburn cells. Vertical paraffin sections of normal (Nil) or irradiated skin at 24hours post-exposure to SSUV (240V). Sunburn cells are shown by arrows. (b) Average number of sunburn cells/linear cm of skin at 0, 24, and 72hours post-exposure to SSUV (240V) and UVA-enhanced radiation (UVB at 180V). Error bars=SD. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Increased UVA/UVB ratio reduces sunburn cell formation. Average number of sunburn cells/cm at 24hours after exposure to increased ratios of UVA/UVB (as UVB voltage was reduced from 240 to 180 and 160V). Error bars=SD. Symbols indicate statistically significantly different treatments. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Increased UVA/UVB ratio decreases epidermal DNA fragmentation. Appearance of DNA fragmentation after exposure to SSUV (240V) and decreased fragmentation with increased ratios of UVA/UVB (as UVB voltage was reduced from 240 to 180 and 160V). Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Formation of caspase-3-positive cells after exposure to UV. (a) Light microscopy of immunohistochemical identification of caspase-3 positivity. Vertical paraffin sections of normal (Nil) or irradiated skin at 24hours post-exposure to 3MEdD of SSUV (240V). Positive cells are stained brown in the epidermal layer. (b) Average number of caspase-3-positive cells/linear cm of skin after exposure to increased ratios of UVA/UVB (as UVB voltage was reduced from 240 to 180 and 160V). Error bars=SD. Symbol indicates statistically significantly different treatments. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Effect of attenuation of SSUV fluence rate on sunburn cell formation. Average number of sunburn cells/linear cm of skin at 24hours post-exposure to 3MEdD of SSUV delivered without spectral change at 240, 180, or 160V. Error bars=SD. No significant difference between treatments. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 Effect of attenuation of SSUV fluence rate on caspase-3-positive cell formation. Average number of caspase-3-positive cells/linear cm of skin at 24hours after exposure to 3MEdD of SSUV delivered without spectral change at 240, 180, or 160V. Error bars=SD. No significant difference between treatments. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

8 Figure 7 Reduced UVB fluence rate has no effect on epidermal thymine dimer formation. Effect of attenuation of the fluence rate of UVB irradiation delivered alone on formation of epidermal thymine dimers at 24hours post-exposure to 3MEdD of UVB. Immunohistochemical detection of DNA dimers in groups of three mice, with UVB voltage reduction from 240 to 180 and 160V, compared with unirradiated skin (Nil), and semiquantitation of average positive nuclei using image analysis. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

9 Figure 8 Inhibition of heme oxygenase activity abrogates UVA protection against sunburn cell formation. Effect of SnPP treatment on average number of sunburn cells/linear cm of skin at 24hours after exposure to increased ratios of UVA/UVB (as UVB voltage was reduced from 240 to 180 and 160V). Error bars=SD. No significant difference between treatments. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

10 Figure 9 Inhibition of heme oxygenase activity decreases UVA protection against caspase-3 positive cell formation. Effect of SnPP treatment on average number of caspase-positive cells/linear cm of skin at 24hours after exposure to increased ratios of UVA/UVB (as UVB voltage was reduced from 240 to 180 and 160V). Error bars=SD. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

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