1. TsNAC1 regulated the growth of T. halophila.
TsNAC1 regulated the growth of T. halophila. A, Diagrams of the plant overexpression vector and RNAi vector. B, Phenotypes of TsNAC1 transgenic plants. Shown are seedlings grown on Murashige and Skoog-agar for 4 d after sowing and plants grown in soil for 6 weeks and 14 weeks (after vernalization treatment for 5 weeks). C, Western-blot results of transgenic lines and the wild type (WT). D, Phenotypes of OX, wild-type, and NR T. halophila plants after 48 h of treatment at −4°C with 3,500 lx, after 1 week of drought stress with an 18% PEG6000 solution, after keeping the 600 mm NaCl concentration of culture medium for 2 weeks, and after 36 h of active oxygen stress with 0.1 mL of 3 mm paraquat solution for every pot. E, TsNAC1 expression levels in OX7, OX10, NR10, NR11, and the wild type (the expression was normalized with Actin), and the dry weight of shoots and roots. Bars represent means ± sd, with three biological replicates in the experiment and five plants for each repeat. Significant differences by Student’s t test are indicated with asterisks: **, P < 0.01.
Can Liu et al. Plant Physiol. 2017;176:
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