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Gene expression (signal intensity) Control Osmotic Salt Drought Root 0 200 400 600 800 Control 0.5 13 612 24 Gene expression (signal intensity) Treatment.

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Presentation on theme: "Gene expression (signal intensity) Control Osmotic Salt Drought Root 0 200 400 600 800 Control 0.5 13 612 24 Gene expression (signal intensity) Treatment."— Presentation transcript:

1 Gene expression (signal intensity) Control Osmotic Salt Drought Root 0 200 400 600 800 Control 0.5 13 612 24 Gene expression (signal intensity) Treatment time (h) 200 400 600 800 Control 0.5 13 612 24 Treatment time (h) Fig. S1. Analysis of bHLH122 responses to various abiotic stresses using the publically available Arabidopsis eFP Browser microarray database (http://www.bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi). Error bars represent means ± SE ( n=3).http://www.bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi

2 0 3 6 9 12 Col 35::bHLH122-13 35S::bHLH122-1 160160 180180 NaCl (mM) Control Biomass (mg plant -1 ) Col 35S::bHLH122 #13#1 Col 35S::bHLH122 #13#1 Control NaCl (160 mM) (a) (b) Fig. S2. Effect of NaCl on the growth of Col-0 and 35S::bHLH122 seedlings. (a) After 96 h of stratification, seeds of different genotypes were grown in a vertical position for 4 days on MS medium and then transferred to medium containing various concentrations of NaCl for an additional 10 days in a vertical position. The photographs show representative seedlings. (b) Biomass of Col-0 and 35S::bHLH122 plants in response to NaCl. Error bars represent means ± SE of approximately 60 plants from three independent experiments

3 Col 35S::bHLH122-13 35S::bHLH122-135S::bHLH122-15 Control Col 35S::bHLH122-13 35S::bHLH122-135S::bHLH122-15 Mannitol (200 mM) Germination (%) 0 20 40 60 80 100 120 100200300 Col 35S::bHLH-13 35S::bHLH-1 35S::bHLH-15 Control Mannitol (mM) A B Fig. S3. Effects of mannitol on the germination and growth of Col-0 and 35S::bHLH122 plants. (a) Effects of mannitol on the seedling growth of Col-0 and 35S::bHLH122 plants on MS medium containing 200 mM mannitol. Seeds were germinated and grown for 10 days. The photographs show representative seedlings. (b) Germination was assessed 96 h after the end of stratification. Error bars represent means ± SE of approximately 150 seeds from three independent experiments.

4 0 5 10 15 20 At4g09760 0 2 4 6 Col #13#1 35S::bHLH122 #15 Relative expression At5g15450 0 0.3 0.6 0.9 1.2 At5g57560 Col #13#1 35S::bHLH122 #15 0 1 2 3 4 5 At3g14205 Col #13#1 35S::bHLH122 #15 0 1 2 3 Relative expression Col #13#1 35S::bHLH122 #15 At5g64940 0 0.3 0.6 0.9 1.2 At4g17490 Col #13#1 35S::bHLH122 #15 Col #13#1 35S::bHLH122 #15 Fig. S4. Analysis of transcript levels in the Col-0 and 35S::bHLH122 transgenic plants. Real-time RT-PCR was used to analyze the expression levels of indicated loci. Real-time RT-PCR quantifications were normalized to the expression of Tub4. Error bars represent means ± SE (n=3).

5 At3g14205 At4g17490 At5g64940 Fig. S5. Schematic diagrams of G-box/E-box motifs in promoter fragments evaluated in ChIP assays. The region between two arrows denoted the amplified fragments in ChIP

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