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Susan L. Prescott, PhD, MD, Paul Noakes, BSc (Hons), PhD, Bonita W. Y

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Presentation on theme: "Susan L. Prescott, PhD, MD, Paul Noakes, BSc (Hons), PhD, Bonita W. Y"— Presentation transcript:

1 Presymptomatic differences in Toll-like receptor function in infants who have allergy 
Susan L. Prescott, PhD, MD, Paul Noakes, BSc (Hons), PhD, Bonita W.Y. Chow, BSc (Hons), Liza Breckler, BSc (Hons), Catherine A. Thornton, BSc (Hons), PhD, Elysia M. Hollams, BSc (Hons), PhD, May Ali, BSc (Hons), Anita H.J. van den Biggelaar, BSc (Hons), PhD, Meri K. Tulic, BSc (Hons), PhD  Journal of Allergy and Clinical Immunology  Volume 122, Issue 2, Pages e5 (August 2008) DOI: /j.jaci Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 A-E, The relationship between maternal allergy and neonatal TLR responses. Cord blood mononuclear cell cytokine responses after TLR activation (as per the Methods section) are shown for neonates with maternal allergy (solid bars, n = 59) and those without maternal allergy (open bars, n = 52). Levels are displayed as geometric means and 95% CIs derived for log-transformed data. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 A-E, The relationship between neonatal TLR responses and subsequent infant allergic disease. Neonatal cytokine responses to TLR activation are shown (geometric mean + 95% CI) for those with subsequent allergic disease at 1 year of age (solid bars, n = 32) and those without maternal allergy (open bars, n = 75). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Comparison of TLR4 expression in allergic and nonallergic groups. The expression of neonatal TLR4 mRNA (geometric mean + 95% CI) is shown at rest (baseline unstimulated) and after LPS stimulation. Levels are compared according to maternal allergy (A) and infant allergy (B). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Comparison of TLR expression (detected by FACS) in high- versus low-cytokine responders to TLR ligation. Neonates were randomly selected for FACS studies on the basis of their responder phenotype, and high responders (>75% percentile; solid bars, n = 5) were compared with low responders (<25% percentile; open bars, n = 5). Cytokine responses are shown (A), together with the TLR expression on each cell population as both percentage of cells expressing each TLR in comparison with negative control, which was set at 0% (B), and the MFI for the TLR+ cells in each subset (C). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 The relationship between neonatal TLR responses and the presence of pets in the maternal home. Neonatal IFN-γ responses to TLR activation are shown (geometric mean + 95% CI) for those with pets in the maternal home (solid bars, n = 40) and those without pets (open bars, n = 67). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 A representative example of high-responder TLR2 expression in different cell types. The relative intensity of TLR2 expression in monocytes (blue), mDCs (red), pDCs (purple), and NK cells (green) in relation to one another and to negative unstained control (shaded) is shown. Results illustrate almost all monocytes to be TLR2+ (99.9% total cells), followed by mDCs (87.5%) and a smaller but significant detection of TLR2 in pDCs (1.64%) and NK cells (2.37%). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 The relationship between neonatal polyclonal T-cell responses and subsequent allergic disease. Neonatal TH1 (IFN-γ) and TH2 (IL-13) responses to polyclonal activation with PHA are shown for neonates with subsequent allergic disease (solid bars, n = 32) and those without allergy (open bars, n = 75). Levels are displayed as geometric means and 95% CIs. Significant differences between the groups are shown. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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