1. Liquid Nitrogen and Cryogenics Training Program
Prepared by: Environmental Health and Safety

2. Potential Hazards Health:
Vapors may cause dizziness or asphyxiation without warning.
Vapors from liquefied gas are initially more dense than air and spread along ground.
Contact with gas or liquefied gas may cause burns, severe injury, and/or frostbite.

3. Public Safety
Immediately call emergency personnel in the event of a large spill or leak.
9-1-1 from campus phone or for ISUPD dispatcher
Isolate large spills or leaks for at least 100 meters in all directions.
Keep unauthorized personnel away.
Stay upwind.
Keep out of low areas.
Ventilate closed spaces before entering.

4. Container Requirements
Closed container with a loose fitting top that allows venting.
Use only vessels approved to contain liquid nitrogen.
Vessel must have carrying handles or be on wheels.

5. Potential Hazards Explosion:
Liquid Nitrogen must be transported and stored in an appropriate container containing pressure relief devices.
Otherwise, when the pressure builds up,
the stopper could be "rocketed out"
or worse, the container could explode

6. Appropriate Containers
Examples of acceptable cryogenic Dewars:

7. Inappropriate Containers
DO NOT use open, un-insulated or glass containers!

8. Liquid Nitrogen Tank Located off loading dock at Science Lab Building
Key can be checked out at the Chemistry storeroom.
Only trained personnel should dispense Liquid Nitrogen.
Enter through South East gate.
Valve Location

9. Liquid Nitrogen Tank
Do NOT touch any of the valves on the North side of the tank.
Do NOT enter through the North gate.
DO NOT touch!

10. Protective Clothing
Always wear thermal protective clothing when handling refrigerated/cryogenic liquids or solids.
It is required you wear a full face shield over safety glasses, loose fitting gauntlet gloves, long sleeve shirts, full-length trousers without cuffs, and fully enclosed shoes.

11. Fill Hose
The liquid Nitrogen is released through this hose from the outside tank.

12. Fill Hose
The liquid Nitrogen is released through this hose from the large inside dewar.

13. Procedures: Step 1: Fill Hose
Insert and hold the fill hose into the appropriate container or into the funnel.

14. Procedures: Step 2: Open/Close Valve
Open valve slowly to begin filling.
Close valve tightly after filling.
Valve
Valve

15. Safe Handling
Always wear personal protective equipment while handling liquid nitrogen.
Always use Liquid Nitrogen in a well ventilated area.
Use a loose-fitting stopper or lid that allows venting of the boil off.
If using a funnel, use caution not to overfill the container.

16. Safe Handling Continued
Only transport liquid nitrogen in the freight elevator with no other occupants present.
Only use containers that have been designed specifically for cryogenic liquids.
Do not store in a confined space.
Do not store at temperatures above 125°F.

17. References DOT Emergency Response Guide CAMEO Chemicals Datasheet
Praxair Liquid Nitrogen Safety Data Sheet