1. Dissecting Regulatory Networks in Host-Pathogen Interaction Using ChIP-on-chip Technology 
Claudia Sala, David C. Grainger, Stewart T. Cole 
Cell Host & Microbe 
Volume 5, Issue 5, Pages (May 2009)
DOI: /j.chom
Copyright © 2009 Elsevier Inc. Terms and Conditions

2. Figure 1 Schematic of the ChIP-on-chip Technology
Cells are crosslinked with formaldehyde in order to introduce reversible covalent bonds between proteins and DNA. After cell lysis and shearing of DNA, the protein of interest is immunoprecipitated together with bound DNA fragments, which are then decrosslinked, purified, labeled, and hybridized to microarrays. Finally, after scanning, ChIP-on-chip signals are plotted against genome coordinates to visualize the protein binding sites.
Cell Host & Microbe 2009 5, DOI: ( /j.chom )
Copyright © 2009 Elsevier Inc. Terms and Conditions