1. Human triple‐negative breast cancers (TNBC) express WNT10B, show active Wnt signalling and have high proliferation, and WNT10B has clinical relevance and prognostic value.A, B.Immunohistochemical (IHC) analysis of tissue microarrays of different breast cancer subtypes shows predominant WNT10B expression in TNBC (quantifications are shown in B).C.TNBC tumours were analysed by IHC for WNT10B, β‐catenin and Ki‐67 expression.
Human triple‐negative breast cancers (TNBC) express WNT10B, show active Wnt signalling and have high proliferation, and WNT10B has clinical relevance and prognostic value.A, B.Immunohistochemical (IHC) analysis of tissue microarrays of different breast cancer subtypes shows predominant WNT10B expression in TNBC (quantifications are shown in B).C.TNBC tumours were analysed by IHC for WNT10B, β‐catenin and Ki‐67 expression. AXIN2 (a Wnt/β‐catenin target gene) mRNA levels were determined by in situ hybridization (ISH). Arrows indicate a similar localization for IHC and ISH. Ten times magnifications are shown. Inserts show 40× magnifications.D.High WNT10B expression in TNBC is significantly correlated with large tumour size and high nuclear grade (WNT10B expression was determined by IHC in TNBC patient samples and results were correlated with patient data). Associations between markers and clinical parameters that were measured on a continuous or ordinal scale were evaluated using Kendall's Tau coefficient. Associations between markers and binary clinical parameters were tested using an exact trend test, associations with nominal parameters were tested using Fisher's exact test. p Values and patient numbers (n) are indicated in the table. Additional information can be found in Supporting Information Fig S7 and the Materials and Methods Section.E, F.Kaplan–Meier survival analysis showing significantly improved recurrence‐free survival of basal‐like breast cancer patients with tumours that express lower levels of WNT10B (E) but not WNT1 (F). Patients with higher (red) and lower expression (black) are indicated as well as numbers of patients at risk at specific time points (below each diagram). Hazard ratios (HR) and p values (log rank p) are depicted for each survival analysis. Kaplan–Meier survival data were generated using the publicly accessible online tool KM‐plotter. p Values of <0.05 were considered to be statistically significant (D–F).
Peter Wend et al. EMBO Mol Med. 2013;5:
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