1. Proliferating Endothelial Cells and Leukocyte Infiltration as Prognostic Markers in Colorectal Cancer 
Coen I.M. Baeten, Karolien Castermans, Harry F.P. Hillen, Arjan W. Griffioen 
Clinical Gastroenterology and Hepatology 
Volume 4, Issue 11, Pages (November 2006)
DOI: /j.cgh
Copyright © 2006 AGA Institute Terms and Conditions

2. Figure 1
Angiogenic parameters in CRC. Relationship between MVD (A) and proliferating ECs (B) to Dukes stage (* represents statistical difference from Dukes B value, P < .05). (C, D) CD31/34 (blue) and ki-67 (brown) double staining of CRC tissues. Note the higher amount of proliferating ECs in D as compared with C, whereas equivalent amounts of blood vessles are present (scale bars, 50 mm). (E) Correlation (and signifcance) between VEGF, MVD, and proliferating ECs. (F) The Kaplan-Meier curve is shown for patients with proliferating ECs above or below the mean value. A significantly favorable prognosis is found in patients with lower counts of proliferating ECs (Log Rank, P < .05).
Clinical Gastroenterology and Hepatology 2006 4, DOI: ( /j.cgh )
Copyright © 2006 AGA Institute Terms and Conditions

3. Figure 2
(A) CD45 staining of a Dukes B staged tumor at the tumor host interface, showing the intense infiltration by leukocytes in the peritumoral area. The dashed line indicates the tumor border. Scale bar represents 100 μm. (B) A higher magnification of a Dukes B staged tumor at an intratumoral area, indicating the difference between leukocyte infiltration in the tumor stroma and in between the tumor cell nests. Stromal leukocytes area is marked by arrowheads, and leukocyte infiltration between the tumor cells is marked with arrows. Scale bar represents 20 μm.
Clinical Gastroenterology and Hepatology 2006 4, DOI: ( /j.cgh )
Copyright © 2006 AGA Institute Terms and Conditions

4. Figure 3
Relationship between leukocyte infiltration and patient survival. Kaplan-Meier curves of patient groups separated on basis of high or low infiltration by CD3, CD8, and CD16 leukocyte subsets. Curves represent leukocyte count areas within the tumors. (A–C) Leukocyte counts within the tumor cell nests in CRC. (D–F) Leukocyte scores in the stroma of CRC. Significance was assesed by Log Rank tests.
Clinical Gastroenterology and Hepatology 2006 4, DOI: ( /j.cgh )
Copyright © 2006 AGA Institute Terms and Conditions

5. Figure 4
Improved survival by combining leukocyte infiltration and angiogenesis parameters. (A) The patient group divided according to both leukocyte infiltration and proliferating ECs into 4 groups. (B) Kaplan-Meier survival curves of the patient groups as defined in panel A. Five-year survival is best in the group with high numbers of infiltrated leukocytes and low numbers of proliferating ECs (group I) and worst in the group with low numbers of infiltrated leukocytes and high numbers of proliferating ECs (group IV, P < .0061).
Clinical Gastroenterology and Hepatology 2006 4, DOI: ( /j.cgh )
Copyright © 2006 AGA Institute Terms and Conditions