1. Questionnaire & physical exam
Timeline
Questionnaire & physical exam
Serum
Dust sample
Blood RNA
Blood DNA
Stool
Meconiun &
Mother
n = 326
n = 200
Newborns
n = 326**
n = 71**
n = 90
Child
(3-6 months)
n = 286*
n = 78
n = 272‡
n = 245
n = 45
(12 months)
n = 280
n = 205
n = 9
n = 272
n = 178
n = 57
(24 months)
n = 274
n = 169
n = 82
n = 153
n = 51
Measurements & Analyses
Demographics,
exposures & clinical outcomes
* (x2) validation of lifestyle +
follow-up
sIgE, IgA IgG levels
**from cord blood
Allergens & endotoxin levels
‡House floors
(n = 232)
child’s bed
(n = 347)
Gene expression
Genotyping
& re-seq
Parasite analysis & Kato Katz
Molecular analysis of gut
microflora
Figure 3. Timeline of data and sample collection activities in the cohort from 0 to 24 months