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Fig. 2. dAST using dPCR is robust to the presence of high concentrations of commensal bacteria due to the specificity of NA amplification. dAST using dPCR.

Published byClotilde Roberge Modified over 5 years ago

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Presentation on theme: "Fig. 2. dAST using dPCR is robust to the presence of high concentrations of commensal bacteria due to the specificity of NA amplification. dAST using dPCR."— Presentation transcript:

1 Fig. 2. dAST using dPCR is robust to the presence of high concentrations of commensal bacteria due to the specificity of NA amplification. dAST using dPCR is robust to the presence of high concentrations of commensal bacteria due to the specificity of NA amplification. (A) A cip-susceptible E. coli isolate and (B) a cip-resistant E. coli isolate from the urine of patients diagnosed with UTIs were exposed to cip (1.0 μg/ml) in the presence of varying amounts of Lj, a common urine commensal. Fold changes relative to time 0 were compared as described in (26) and used to determine susceptibility. (C) Susceptibility determined using the CT ratios after 15 min of antibiotic exposure for each concentration of Lj tested. n = 2 technical replicates for each biological sample. Error bars are 98% confidence intervals. Nathan G. Schoepp et al., Sci Transl Med 2017;9:eaal3693 Published by AAAS

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