1. Preceding knockdown of FoxO3 reduces inhibitory effect of UCN‐01 on human lung fibroblast proliferation and myofibroblast transdifferentiation in response to pro‐fibrotic stimuli
Preceding knockdown of FoxO3 reduces inhibitory effect of UCN‐01 on human lung fibroblast proliferation and myofibroblast transdifferentiation in response to pro‐fibrotic stimuli N‐HLF were transfected with scramble siRNA or FoxO3 siRNA. 6 h after transfection, cells were serum‐starved for 36 h and then stimulated with 5% FCS in the presence or absence of UCN‐01 (50 nM), and cell proliferation was measured by BrdU incorporation after 24 h. Scramble siRNA‐transfected cells were left non‐stimulated for 24 h as an additional control. Data represent percentage of control, scramble siRNA non‐stimulated cells (n = 2–3). Data are expressed as mean ± SEM and were analyzed using one‐way ANOVA, **P < 0.01 versus 5% FCS‐scramble siRNA.N‐HLF was transfected with scramble siRNA or FoxO3 siRNA. 6 h after transfection, cells were serum‐starved for 36 h and then stimulated with TGF‐β1 (10 ng/ml) in the presence or absence of UCN‐01 (50 nM) for 24 h and mRNA expression of COL1A1 and COL3A1 were measured with qPCR (n = 3). Data are expressed as mean ± SEM and were analyzed using one‐way ANOVA, n.s. = not significant, **P < 0.01 versus scramble siRNA, §P < 0.05 versus TGF‐β1‐scramble siRNA, #P < 0.05 versus UCN‐01‐scramble siRNA.
Hamza M Al‐Tamari et al. EMBO Mol Med. 2017;emmm
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