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Interleukin 18 binding protein (IL18-BP) inhibits neointimal hyperplasia after balloon injury in an atherosclerotic rabbit model  Jian-Ming Li, MD, Mohammad.

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Presentation on theme: "Interleukin 18 binding protein (IL18-BP) inhibits neointimal hyperplasia after balloon injury in an atherosclerotic rabbit model  Jian-Ming Li, MD, Mohammad."— Presentation transcript:

1 Interleukin 18 binding protein (IL18-BP) inhibits neointimal hyperplasia after balloon injury in an atherosclerotic rabbit model  Jian-Ming Li, MD, Mohammad H. Eslami, MD, Michael J. Rohrer, MD, Phong Dargon, MD, Isabella Joris, PhD, Gregory Hendricks, PhD, Stephen Baker, MScPH, Bruce S. Cutler, MD  Journal of Vascular Surgery  Volume 47, Issue 5, Pages (May 2008) DOI: /j.jvs Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 A, Immunofluorescence stain of cross-section of rabbit femoral artery 2 weeks and 4 weeks after balloon angioplasty. PMN (red), mono/macro (green), and nucleus (blue). Bar = 5 μm. B, Quantification of inflammatory cell reaction of rabbit femoral artery 2 weeks and 4 weeks after balloon-injury. There were no significant effects of dosage or time on PMN and lymphocytes. For mono/macro IL18-BP 10 μg group was significantly less than control group, and IL18-BP 5 μg and 25 μg groups (P < .0002, < .0002, and = .0036, respectively) at 2 weeks. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Effect of IL18-BP on neointima to media area ratio (I/M ratio), lumen to whole artery area ratio (L/A ratio), and luminal area of the femoral artery 2 weeks and 4 weeks after balloon-injury. Mean ± SEM, N = 3-5. Each bar represents 18 to 30 sections of the left balloon-injured carotid artery from three to five animals. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Blood lipid panel before and after 2 weeks, 4 weeks, and 6 weeks of high fat diet. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 A, Lipid deposition on the inner surface of rabbit descending thoracic aorta with and without IL18-BP after 4 weeks and 6 weeks high fat diet (A, ORO stain); Calcium deposition in the ascending thoracic aorta without IL18-BP after 4 weeks high fat diet (B, HE stain). C, Percentage area of lipid deposition on the inner surface of a segment of the descending thoracic aorta. Data represent the percentage of total lipid deposition area on 4.38 cm2 surface. Two animals of each group were analyzed. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

6 Fig 5 A, ORO staining of cross-section of rabbit descending thoracic aorta. Lipid (red) and nucleus (blue). Lipid laden cells are seen after 6 weeks high fat diet. No lipid laden cell is seen in IL18-BP treatment groups. B, Immunofluorescence stain of cross-section of rabbit descending aorta 6 weeks after high fat diet. Lipid (red), macrophage (green), and nucleus (blue). Upper row, Lipid laden macrophages (↓ black, white), endothelial cells (↓↓), and SMC (asterisk). Bottom row, merged image of two images of upper rows identifying foam cells. C, Transmission electron microscopy (TEM) image of cross-section of rabbit descending aorta 6 weeks after high fat diet without IL18-BP. Two Macrophages are seen that have lipid laden (foam cells), one is undergoing diapedesis between endothelial cells, and the other one beneath endothelium. (×8000). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

7 Fig 5 A, ORO staining of cross-section of rabbit descending thoracic aorta. Lipid (red) and nucleus (blue). Lipid laden cells are seen after 6 weeks high fat diet. No lipid laden cell is seen in IL18-BP treatment groups. B, Immunofluorescence stain of cross-section of rabbit descending aorta 6 weeks after high fat diet. Lipid (red), macrophage (green), and nucleus (blue). Upper row, Lipid laden macrophages (↓ black, white), endothelial cells (↓↓), and SMC (asterisk). Bottom row, merged image of two images of upper rows identifying foam cells. C, Transmission electron microscopy (TEM) image of cross-section of rabbit descending aorta 6 weeks after high fat diet without IL18-BP. Two Macrophages are seen that have lipid laden (foam cells), one is undergoing diapedesis between endothelial cells, and the other one beneath endothelium. (×8000). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

8 Fig 6 Serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) level. Before and after 6 weeks of high fat diet with and without IL18-BP. SGOT was significantly elevated with IL18-BP 25 μg. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

9 Fig 7 A and B, Microphotograph of representative cross-sections of femoral artery after balloon-injury with and without IL18-BP in an atherosclerotic rabbit model. Original magnifications ×4 (A), ×10 (B). HE stain, Bar = 100 microns. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

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