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Engineered Living Blood Vessels: Functional Endothelia Generated From Human Umbilical Cord-Derived Progenitors  Dörthe Schmidt, MD, Lars M. Asmis, MD,

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Presentation on theme: "Engineered Living Blood Vessels: Functional Endothelia Generated From Human Umbilical Cord-Derived Progenitors  Dörthe Schmidt, MD, Lars M. Asmis, MD,"— Presentation transcript:

1 Engineered Living Blood Vessels: Functional Endothelia Generated From Human Umbilical Cord-Derived Progenitors  Dörthe Schmidt, MD, Lars M. Asmis, MD, Bernhard Odermatt, MD, Jens Kelm, PhD, Christian Breymann, MD, Matthias Gössi, PhD, Michele Genoni, MD, Gregor Zund, MD, Simon P. Hoerstrup, MD, PhD  The Annals of Thoracic Surgery  Volume 82, Issue 4, Pages (October 2006) DOI: /j.athoracsur Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

2 Fig 1 Macroscopic appearance of tissue-engineered blood vessels. Vascular scaffolds of 0.5 cm diameter and 3 cm length fabricated from nonwoven polyglycolic acid mesh and poly-4-hydroxybutyric acid before seeding (A) and after in vitro culturing (B). Tissue-engineered blood vessels were intact, pliable, and densely covered with cells (B). The lumen was open, and the wall thickness was homogeneous at about 0.3 cm. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

3 Fig 2 Histology and immunohistochemistry of tissue-engineered blood vessels. (A) Hematoxylin and eosin staining revealed a cellular tissue with an outer layer consisting of loosely arranged cells and a middle layer organized more compactly. The innermost layer consisted of endothelial cells. (B) Cells in the middle layer expressed α-smooth muscle actin. (C) A CD31-positive cell layer lined the lumen of the tissue-engineered blood vessels. Additionally, some cells expressing CD31 could be found in the middle layer. (D) Higher magnification of CD31-positive cells distant from the luminal cell lining. Arrows point at remnants of scaffold. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

4 Fig 3 Morphology of the inner surfaces. Scanning electron micrographs show smooth inner surfaces of the tissue-engineered blood vessels densely covered with endothelial cells derived from endothelial progenitor cells (A) and human umbilical vein endothelial cells (B). The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

5 Fig 4 Quantification of extracellular matrix elements and cell number. Total cell number (represented as DNA) in tissue-engineered blood vessels was 90% of native tissue values; glycosaminoglycans (GAG) and collagen (hydroxyproline, HYP) were 62% and 1%, respectively. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

6 Fig 5 Functional plasticity and bioactivity of endothelial cells. Both endothelial progenitor cell–derived endothelial cells (EPC) and human umbilical vein endothelial cells (HUVEC) expressed CD31 and thrombomodulin (TM) but lacked the expression of tissue factor (TF) (grey). After stimulation with tumor necrosis factor-α (black) the expression of thrombomodulin was decreased whereas the expression of tissue factor was upregulated. CD31 expression was not affected. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

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