1. Volume 23, Issue 3, Pages 221-228 (September 2016)
Dynamics of early stem cell recruitment in skin flaps subjected to ischemia reperfusion injury 
Ya Hui Tang, MD, PhD, Lindsey A. Pennington, MD, Jessica W. Scordino, MD, Jonathan Steven Alexander, PhD, Timothy Lian, MD 
Pathophysiology 
Volume 23, Issue 3, Pages (September 2016)
DOI: /j.pathophys
Copyright © 2016 Elsevier B.V. Terms and Conditions

2. Fig 1
Percent necrosis in postischemic skin flap of control and experimental mice with BMSCs treatment. The percent necrosis decreased significantly in experimental mice comparison with control on day 3, 5 and 7 post I/R. Sham: n=5; Ischemia control: n=7; Ischemia+BMSC: n=6. ** p<0.005.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2016 Elsevier B.V. Terms and Conditions

3. Fig. 2
Macroscopic appearance of control (Sham, left panel), post-ischemic (I/R, middle panel) and BMSC-treated post-ischemic (I/R+BMSC, right panel) skin flaps in mice at Day 3 post-surgery. Yellow dots: Necrosis areas. Scale bar: 5mm (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.).
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2016 Elsevier B.V. Terms and Conditions

4. Fig. 3
Rolling velocity of BMSCs and leukocytes in skin flap venules of control and postischemic (I/R) mice. Control and I/R mice n=6, respectively. * p<0.05 and ** p<0.005 relative to leukocyte.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2016 Elsevier B.V. Terms and Conditions

5. Fig. 4
A: Magnified image of 1×2cm flap mapped on the right abdomen area of a C57 Bl/6J mouse. B: An image of the inferior epigastric vessels (black arrow) pedicled island flap raised with a pedicle clamp in place. Black rectangle: distal area (6×3mm) from edge of incision toward pedicle; Green square: proximal area (3×3mm). C & D: Photomicrographs of a skin flap from a control mouse showing the trafficking of BMSC and leukocytes in situ 2h following injection i.v. of the CFSE labeled BMSC obtained by intravital fluorescent microscopy. C: a BMSC (white arrowhead) adhered to a wall of venule and the other BMSC was rolling within the vessel (direction of flow is indicated by the red arrowhead) of a raised skin flap. D: Many BMSC (white arrow) and leukocytes transmigrated into interstitial of distal area. Black arrow: leukocyte. Black arrowhead: adipocyte. Scale bar=100μm. E: Schematic of time points of I/R, BMSCs injection and microscopy examination. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2016 Elsevier B.V. Terms and Conditions

6. Fig. 5
Flux fraction of BMSCs (rolling number of BMSCs divided by total rolling plus free flow numbers of BMSCs; A) and leukocytes (B) rolling were analysed in skin flap venules of control and postischemic (I/R) mice 2 and 4h post reperfusion. Control and I/R mice n=6, respectively. * p<0.05.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2016 Elsevier B.V. Terms and Conditions

7. Fig. 6
BMSCs adhesion percentage per minute (number of BMSCs adhesion divided by total numbers of flux plus adhesion of BMSCs per minute; A) and leukocytes adhesion percentage per minute (B) were analysed in skin flap venules of control and postischemic (I/R) mice 2 and 4h post reperfusion. Control and I/R mice n=6, respectively.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2016 Elsevier B.V. Terms and Conditions

8. Fig. 7
Transmigrated BMSCs (A) and leukocytes (B) were observed in the interstitial of skin flap of control and postischemic (I/R) mice 2 and 4h post reperfusion. Control and I/R mice n=6, respectively. * p<0.05, ** p< # indicates leukocyte compared to BMSC correspondently p<0.05, ## p<0.005.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2016 Elsevier B.V. Terms and Conditions

9. Fig. 8
Transmigrated BMSCs were observed in the proximal and distal part of skin flap of control and postischemic (I/R) mice 2 (A) and 4h (B) post reperfusion. Control and I/R mice n=6, respectively. * p<0.05, ** p<0.005.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2016 Elsevier B.V. Terms and Conditions