1. Spontaneous Autologous Graft-versus-Host Disease in Plasma Cell Myeloma Autograft Recipients: Flow Cytometric Analysis of Hematopoietic Progenitor Cell Grafts 
Hillard M. Lazarus, Scott R. Sommers, Lisa M. Arfons, Pingfu Fu, S.A. Ataergin, N.M. Kaye, F. Liu, Tamila L. Kindwall-Keller, Brenda W. Cooper, Mary J. Laughlin, Richard J. Creger, Paul M. Barr, Stanton L. Gerson, David Kaplan 
Biology of Blood and Marrow Transplantation 
Volume 17, Issue 7, Pages (July 2011)
DOI: /j.bbmt
Copyright © 2011 American Society for Blood and Marrow Transplantation Terms and Conditions

2. Figure 1
Representative flow cytometric analysis of CD34+ cells in mobilized blood samples. Mononuclear cells from 4 different mobilized blood samples were stained for CD34 expression (upper row). The cells were also stained with control immunoglobulin (middle row) or with specific antibodies (lower row) and processed by EAS for high-resolution immunophenotyping. The specific antibodies shown are CXCR4 (lower left), CD117 (lower middle left), GATA-2 (lower middle right), and Bmi1 (lower right). The stains for CXCR4 and CD117 are surface stains without fixation and permeabilization. The stains for GATA-2 and Bmi-1 are intracellular stains with fixation and permeabilization. The amplified signals (middle and lower rows) are shown only for the CD34+ gates (upper row). Representative results are shown from 4 different donors in order to demonstrate the consistency in CD34+ cell delineation.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2011 American Society for Blood and Marrow Transplantation Terms and Conditions

3. Figure 2
Correlation of cMyc and GATA-2 expression levels in CD34+ cells. CD34+ cells from various samples of mobilized blood from patients with multiple myeloma were stained for the expression of c-MYC and separately for the expression of GATA-2. The cells were processed by EAS for high-resolution immunophenotyping. Samples not associated with the subsequent development of autologous GVHD in the transplanted patients (left panel) and samples that were associated with the subsequent development of autologous GVHD in the transplanted patients (right panel) are shown. The distributions of median fluorescence ratios are shown for the various samples. Coefficients of correlation and P values are shown. Abbreviations: aGVHD indicates acute graft-versus-host disease.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2011 American Society for Blood and Marrow Transplantation Terms and Conditions