1. Bsc (Hons) Biomedical Sciences, UKM
PSEUDOMONAS spp.
Lecture 12
Prepared by:
Miss Norzawani Jaffar,
Bsc (Hons) Biomedical Sciences, UKM

2. Learning Outcomes
At the end of learning session, student must be able to:
Describe characteristic of Pseudomonas sp.
Explain the morphology and classification of Pseudomonas sp.
Describe the laboratory test that significant for Pseudomonas sp identification.
Explain the related disease and infections that potentially cause by Pseudomonas sp.

3. Pseudomonas sp Scientific classification Domain: Bacteria
Phylum: Proteobacteria
Class: Gammaproteobacteria
Order: Pseudomonadales
Family: Pseudomonadaceae
Genus: Pseudomonas

4. Pseudomonas sp Strict aerobes widely distributed in nature
They are mainly opportunist pathogens infecting those with impaired septicaemia.
Some rare GNRs are environmental organism causing hospital-acquired infections from contaminated fluids or equipment.
Other rare Gram negative rods are normal or animal flora causing opportunistic infections including dental infections, infected bites and systemic infections.
Pseudomonal species have been found in soil, water, plants, and animals; Pseudomonas aeruginosa colonization reportedly occurs in more than 50% of humans, and P aeruginosa is the most common pseudomonal species.

5. Characteristic
Members of the genus display the following defining characteristics;
Rod shaped
Gram-negative
One or more polar flagella, providing motility
Aerobic
Non–spore forming
positive catalase test
positive oxidase test.
Beta hemolytic (on blood agar)
Indole negative
Methyl red negative
Citrate positive.

6. Pseudomonas colonies on agar plate

8. Pseudomonas with flagella

9. Diseases

10. Pseudomonal infection, as described by Pollack, occurs in 3 stages;
(1) bacterial attachment and colonization, followed by
(2) local invasion
(3) dissemination and systemic disease

11. End…….. Q & A