3. (new bacteria are clones of original bacteria)
Recombinant DNA
(new bacteria are clones of original bacteria)

5. Therapeutic
Cloning

6. 9:00 Gel Electrophoresis
Restriction enzymes cut DNA into different sized fragments so scientists can study different genes.
DNA fragments move down the gel, toward the + charge.
Smaller DNA fragments move farther down the gel.

8. What Do You Know? Peer-To-Peer Discourse+

9. 1. What is this called?
2. What is being separated? What made the cuts?
3. What charge does DNA have?
4. Which lane has the least dense (smallest) DNA?
5. Which lane has the most dense (longest) DNA?
6. If Lane 1 is the evidence from the crime scene,
who committed the crime?
Lane 1 - +
Lane 6

10. 12:00- What Do You Know? Peer-to-Peer Discourse

11. 1. What is the process called when genes are inserted into an organism?
2. What are the small rings of DNA called that are used in this process and found in bacteria?
3. What kind of enzyme cuts the DNA? Pastes the DNA back together?
4. Why do you think this newly created plasmid is called recombinant DNA?

12. 3:00- What Do You Know? Peer-To-Peer Discourse

13. Arrange them sequentially in the order they are used.
Summarize the procedure, the events, and the outcome.

14. Comparative writing
Restriction enzymes are used to remove the FP gene from jellyfish or coral.
The same restriction enzyme is used to cut the plasmid (circular piece of DNA from bacteria) and then insert the FP gene into it.
Next the transformed plasmid is inserted into E. coli bacteria.
The bacteria are streaked across an LB Agar (nutrient) plate, and allowed time to carry out Binary Fission (dividing as much as 106 overnight).
The result is many bacteria now expressing the FP gene, making Fluorescent Proteins, in a procedure called Protein Transformation

15. In this example, what is the source organism?
What is the function of the Bt gene?
Into what organism is this Bt gene inserted? Why?

16. 4. Name two other reasons scientists might want to genetically modify plants.