Presentation is loading. Please wait.

Presentation is loading. Please wait.

Cornelis J. J. Huijsmans, Jeroen Poodt, Paul H. M. Savelkoul, Mirjam H

Similar presentations


Presentation on theme: "Cornelis J. J. Huijsmans, Jeroen Poodt, Paul H. M. Savelkoul, Mirjam H"— Presentation transcript:

1 Sensitive Detection and Quantification of the JAK2V617F Allele by Real-Time PCR 
Cornelis J.J. Huijsmans, Jeroen Poodt, Paul H.M. Savelkoul, Mirjam H.A. Hermans  The Journal of Molecular Diagnostics  Volume 13, Issue 5, Pages (September 2011) DOI: /j.jmoldx Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Standard curves (using twofold dilution steps) generated by JAK2V617F real-time PCR using PNA blocking (squares) and LNA blocking (triangles). Data are given as mean Ct ± SD. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 Amplification plots of JAK2V617F real-time PCR of 25 (A) and 22 (B) healthy individuals in duplicate (JAK2V617F negative cohort) and one 1% JAK2V617F positive control. The amplification plots shown are representative of the entire cohort. Gray horizontal line (at Delta Rn 0.8e-002) indicates threshold; black vertical line, cutoff CT value of 37.9. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions


Download ppt "Cornelis J. J. Huijsmans, Jeroen Poodt, Paul H. M. Savelkoul, Mirjam H"

Similar presentations


Ads by Google