1. Rotating Vessel Wall For the Adhesion of Cardiac Cells
Roli Kumar
BME Oral Presentation#1
Advisor: Dr. Marc Lin

2. Background
Many surgical and radiational procedures have left areas of damaged tissue in the heart.
Test new drugs
study development and function of cardiac tissue

3. Objectives 3-D cardiac tissue from a monolayer of cardiac cells
3-D cell cultures contain specifications similar to in vivo:
cell to cell and cell to matrix interactions
composition of interstitial fluid
Three dimensional Cultures of Prostatic Cells

4. Design Specifications of Rotating Vessel
End over end mixing of the cells
Continuous rotation of the vessel
Max of .2 dyne/cm2 of shear stress
Minimal fluid turbulence
Bubble-free aeration
eliminate hydrodynamic forces
Solid Body Rotation
Suspending the cells in a liquid medium.

5. Apparatus Set-Up
Plastic Rod
Rotating vessel
Water
Magnetic Stirrer

6. Work Completed Literature search was completed: Found a metal ball
Cell disassociation
Rotating-Wall Vessel for Prostate Cells
Found a metal ball

7. Current Work
I am meeting with Dr. Bader who does Cardiology research to learn how to disassociate and cardiac cell growth.
Putting the apparatus together

8. Future Plan of Action
Calculate the sheer stress applied on cells when rotated
Literature search on a way to bubble-free aeration of cells while they are rotating