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The effect of estrogen on diabetic wound healing is mediated through increasing the function of various bone marrow-derived progenitor cells  Ying Zhuge,

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Presentation on theme: "The effect of estrogen on diabetic wound healing is mediated through increasing the function of various bone marrow-derived progenitor cells  Ying Zhuge,"— Presentation transcript:

1 The effect of estrogen on diabetic wound healing is mediated through increasing the function of various bone marrow-derived progenitor cells  Ying Zhuge, MD, Manuela M. Regueiro, PhD, Runxia Tian, MD, Yan Li, BS, Xiaomei Xia, MD, Roberto Vazquez-Padron, PhD, Sharon Elliot, PhD, Seth R. Thaller, MD, Zhao-Jun Liu, PhD, Omaida C. Velazquez, MD  Journal of Vascular Surgery  Volume 68, Issue 6, Pages 127S-135S (December 2018) DOI: /j.jvs Copyright © 2018 The Authors Terms and Conditions

2 Fig 1 Topical estrogen treatment does not affect the numbers of bone marrow-derived endothelial progenitor cells (BM-EPCs) in wounded diabetic mice. Data are presented as percentage of BM-EPCs from three replicates. NS, Not significant. N = 6 mice/group. Journal of Vascular Surgery  , 127S-135SDOI: ( /j.jvs ) Copyright © 2018 The Authors Terms and Conditions

3 Fig 2 Estrogen treatment improves the viability and proliferation of bone marrow-derived endothelial progenitor cells (BM-EPCs) and bone marrow-derived mesenchymal stem cells (BM-MSCs) in vitro. a, Diabetic db+/db+ mice had significantly fewer colony-forming units (CFUs) of EPCs than nondiabetic db+/db− mice (*; P = .03), and this deficit was partially rescued by topical estrogen treatment (**; P = .013). b, Similarly, diabetic db+/db+ mice had fewer CFUs of MSCs than nondiabetic db+/db− mice (*; P = .001), which was also partially rescued by topical estrogen treatment (**; P = .03). Data are presented as mean ± standard deviation of three replicates. N = 6 mice/group. Journal of Vascular Surgery  , 127S-135SDOI: ( /j.jvs ) Copyright © 2018 The Authors Terms and Conditions

4 Fig 3 Topical estrogen treatment promotes diabetic wound healing. A significant wound healing advantage is observed for estrogen-treated diabetic wounds starting as early as day 6. a, Representative wounds at different days are shown for each group. b, Wound healing rate is expressed as percentage recovery (*; P = .04). N = 6 mice/group. DAPI, 4′,6-Diamidino-2-phenylindole; EPCs, endothelial progenitor cells; FITC, fluorescein isothiocyanate. Journal of Vascular Surgery  , 127S-135SDOI: ( /j.jvs ) Copyright © 2018 The Authors Terms and Conditions

5 Fig 4 A proangiogenic effect of estrogen treatment on diabetic wound neovascularization. In comparison to diabetic wounds treated with placebo, estrogen-treated wounds had denser neovascularization in the wound bed and the area immediately surrounding the wound. a, Representative confocal laser microscopy images. b, Quantitation of vessel density from four randomly selected fields (magnification ×40) in each group (*; P = .003). N = 6 mice/group. Journal of Vascular Surgery  , 127S-135SDOI: ( /j.jvs ) Copyright © 2018 The Authors Terms and Conditions

6 Fig 5 Estrogen treatment enhances recruitment of endothelial progenitor cells (EPCs) to diabetic wounds. Wound blood vessel perfusion with DiI dye. a, Representative images of DiI-stained wound blood vessels measured by laser scanning confocal microscopy at day 15 are shown for each group. b, Quantification of vessel density in the wounds. Percentage of threshold area covers all vessels detected as a percentage of the entire wound area (*; P = .036 on day 6, .023 on day 7, .005 on day 9, .022 on day 11, and .067 on day 13). N = 6 mice/group. Journal of Vascular Surgery  , 127S-135SDOI: ( /j.jvs ) Copyright © 2018 The Authors Terms and Conditions

7 Fig 6 Estrogen treatment increases collagen deposition in diabetic wounds. Increased collagen deposition as measured by the scar density in estrogen-treated wound tissue was observed at day 15. a, Representative images of laser scanning confocal microscopy at day 15 are shown for each group. b, Quantification of scar density in the wounds. Percentage of threshold area covers all scars detected as a percentage of the entire wound area minus stained blood vessels (*; P = .04). N = 6 mice/group. Journal of Vascular Surgery  , 127S-135SDOI: ( /j.jvs ) Copyright © 2018 The Authors Terms and Conditions

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