1. O2 CO2 RESPIRATORY ENZYMES ATP Catalaze H202 ENERGY
Superoxide dismutase O2
H202
Amal Ali ALmousa 140 mic
30/03/1440

2. Anaerobies Aerobes Obligate aerobes Microaerophiles
Facultative anaerobes
Microaerophiles
Obligate aerobes
Obligate anaerobes
Aerotolrant anaerobes
Amal Ali ALmousa 140 mic
30/03/1440

3. Microaerophiles Facultative anaerobes Obligate anaerobes
Aerotolrant anaerobes
Obligate aerobies
Amal Ali ALmousa 140 mic
30/03/1440

4. Obligate anaerobes in lab
Amal Ali ALmousa 140 mic
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5. Balanced growth Growth cycle: Lag phase Log phase Stationary phase
Death phase
The Growth Curve
Amal Ali ALmousa 140 mic
30/03/1440

6. Measuring Growth 1-Cell Count Direct Measurement
Direct Microscopic Count
In direct Measurement
colony counting
hemocytometer
Amal Ali ALmousa 140 mic
30/03/1440

7. 3-Measurement of enzymatic activity In direct Measurement
2-Mass determination Direct Measurement weighed (wet mas). dried weighed( dry mass In direct Measurement Turbidity (
3-Measurement of enzymatic activity In direct Measurement
Amal Ali ALmousa 140 mic
30/03/1440

8. Factors affecting microbial growth
1-Physical factors 2-Chemical factors 3-Biological factors
Amal Ali ALmousa 140 mic
30/03/1440

9. 1-Physical factors
Temperature O2 pH Osmotic Pressure radiation Moisture Desication Surface tension atmospheric pressure
Amal Ali ALmousa 140 mic
30/03/1440

10. 1-Temperature Minimum growth temperature Optimum growth temperature
Maximum growth temperature
Amal Ali ALmousa 140 mic
30/03/1440

11. 2-Oxygen concentration slid 2
3- pH value Acidophilus :pH 0 and 5.5 Neutrophilus : pH 5.5 and 8 Alkalophileus :pH 8 – 11.5
Amal Ali ALmousa 140 mic
30/03/1440

12. :4-Osmotic Pressure A-Hypotonic B-Hypertonic Plasmolysis C-Isotonic
Amal Ali ALmousa 140 mic
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13. UV rays, Ionizing radiation ))5- radiation
6-Moisture (Water activity)
7-Desiccation
Amal Ali ALmousa 140 mic
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14. 9- atmospheric pressure
8-Surface tension
9- atmospheric pressure
Amal Ali ALmousa 140 mic
30/03/1440

15. 2-Chemical factors 1-Germistatic agents 2-Germicidal agents
Amal Ali ALmousa 140 mic
30/03/1440

16. Microbes group RELATIONS
3-Biological factors
Microbes group
RELATIONS
Amal Ali ALmousa 140 mic
30/03/1440