1. Letrozole increases ovarian growth and Cyp17a1 gene expression in the rat ovary 
Israel Ortega, M.D., Anna Sokalska, M.D., Ph.D., Jesus A. Villanueva, Ph.D., Amanda B. Cress, B.S., Donna H. Wong, Ph.D., Elisabet Stener-Victorin, Ph.D., Scott D. Stanley, Ph.D., Antoni J. Duleba, M.D. 
Fertility and Sterility 
Volume 99, Issue 3, Pages (March 2013)
DOI: /j.fertnstert
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Growth curves of rats exposed to placebo (control, n = 14) or letrozole (n = 14) from birth to 92nd day of age. Pellets were implanted at 21 days of age. Each point represents mean ± SEM. *P<.001 vs. control.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Ovarian morphology of (A) control rats and (B) letrozole-treated rats. Ovarian sections were stained with hematoxylin and eosin and analyzed under a conventional microscope (original magnification, ×400). Theca-interstitial cell layers are indicated by yellow arrows.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
(A) Effect of letrozole on (a) A, (b) T, and (c) LH levels in plasma and serum samples. Androgen levels were determined using liquid chromatography–mass spectrometry. Luteinizing hormone levels were measured in serum by a sensitive two-site sandwich immunoassay. Results are presented as a percentage of control. Each bar represents the mean ± SEM. **P<.001 vs. control. (B) Effect of letrozole on mRNA expression of (a) Star, (b) Cyp11a1, (c) Hsd3b1, and (d) Cyp17a1 in rat ovaries. Total cellular RNA was isolated, and mRNA expression was determined using quantitative real-time PCR reactions and normalized to Hprt mRNA levels. Results are presented as a percentage of control. Each bar represents the mean ± SEM. *P<.05 vs. control; **P<.001 vs. control.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions