1. Figure 2 Use of CRISPR/Cas9 for genome editing
Figure 2 | Use of CRISPR/Cas9 for genome editing. a | CRISPR/Cas9 generates a double-strand break at a target site in the genome. If the cell uses error-prone non-homologous end-joining to repair the break, it can introduce indels. If the target site is within the early coding sequence of a gene, the indels can represent frameshift mutations that effectively knock out the gene. b | If an exogenous DNA repair template is introduced into the cell, the cell can use homology-directed repair to repair the break, thus inserting a mutation into the genome. This same approach can be used to correct a mutation. However, non-homologous end-joining will occur in parallel and introduce indels at the target site in some cells, usually with higher efficiency than knock-in of the desired mutation. bp, base pair; PAM, protospacer-adjacent motif.
Strong, A. & Musunuru, K. (2016) Genome editing in cardiovascular diseases
Nat. Rev. Cardiol. doi: /nrcardio