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Rewiring Cas9 to Target New PAM Sequences

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Presentation on theme: "Rewiring Cas9 to Target New PAM Sequences"— Presentation transcript:

1 Rewiring Cas9 to Target New PAM Sequences
Virginijus Siksnys, Giedrius Gasiunas  Molecular Cell  Volume 61, Issue 6, Pages (March 2016) DOI: /j.molcel Copyright © 2016 Elsevier Inc. Terms and Conditions

2 Figure 1 Structural Mechanism of a Protospacer Adjacent Motif Sequence Recognition by SpCas9 The DNA target site for SpCas9 is composite and consists of two nucleotide sequences termed a protospacer and protospacer adjacent motif (PAM), respectively. Protospacer interaction motif (PIM) recognizes the 5′-GG-3′ PAM in the WT SpCas9. Mutations in PIM sequence enable recognition of altered PAM by an induced fit mechanism. Molecular Cell  , DOI: ( /j.molcel ) Copyright © 2016 Elsevier Inc. Terms and Conditions

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