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In vitro modification by SUMO‐1 and SUMO‐2 of HDACs and HDAC‐related proteins. 35S‐labelled in vitro translated HDAC1, HDAC3, HDAC6 and MITR were incubated.

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Presentation on theme: "In vitro modification by SUMO‐1 and SUMO‐2 of HDACs and HDAC‐related proteins. 35S‐labelled in vitro translated HDAC1, HDAC3, HDAC6 and MITR were incubated."— Presentation transcript:

1 In vitro modification by SUMO‐1 and SUMO‐2 of HDACs and HDAC‐related proteins. 35S‐labelled in vitro translated HDAC1, HDAC3, HDAC6 and MITR were incubated in a sumoylation mix containing a fraction of HeLa cells providing the E1 activity together with recombinant Ubc9, in either the absence (MIX) or presence of recombinant SUMO‐1 or SUMO‐2. In vitro modification by SUMO‐1 and SUMO‐2 of HDACs and HDAC‐related proteins. 35S‐labelled in vitro translated HDAC1, HDAC3, HDAC6 and MITR were incubated in a sumoylation mix containing a fraction of HeLa cells providing the E1 activity together with recombinant Ubc9, in either the absence (MIX) or presence of recombinant SUMO‐1 or SUMO‐2. The positions of unmodified and modified substrate proteins are indicated. Olivier Kirsh et al. EMBO J. 2002;21: © as stated in the article, figure or figure legend

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