1. A Modern Breakthrough For Life's Most Precious Resource:
Hydro Enterprises, Inc.
A Modern Breakthrough For Life's Most Precious Resource:
Water
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2. Plasma Activated Water Technology
Will be considered one of the most modern day marvels of this century.
Our Plasma Activated Water Technology will make water monomolecular, remove the harmful effects of bacteria, viruses and other pollutants created by man, and if you desire can enhance the structure of water for your benefit. .
This is done with….
> NO Chemicals
> Little or NO Salts
> NO Harmful Processes
Natures Most Precious Resource - Water 2

3. Plasma Activated Water (PAW) Technology?
Primary Benefits
Makes water monomolecular in structure. The monomolecular structure is stable for a sufficient period of time to be used for thousands of applications.
Provides for the ability to kill bacteria and viruses contained in water or fluids. Several tests have been completed including wastewater treatment tests, that proved 99.9% kill within the water being processed.
Provides for the ability to kill any bacteria and virus on any surface upon contact. The actual time to obtain a 6 log kill is on contact, not in seconds. Several tests have proven a 99.9% kill of bacteria and viruses such as e-coli, bacillus subtilis, staph, poliovirus, and rotavirus.
The PAW Technology has the ability to facilitate the removal of contaminants from any water source.
Provides the ability to make High pH water, up to 12.5 from any water source with an Oxygen Reduction Potential (ORP) of -600 mV and Low pH water as low as 1.5 with an ORP of + 1,200 mV. 3

4. Plasma Activated Water Technology Agriculture
Will be considered one of the most modern day marvels of this century.
Agriculture
Crops – Dairy - Poultry – Beef – Pork
Production and Processing
Improves plant and animal growth rates
Eliminates / Reduces contaminates
Extends product shelf life by 7 to 14 days.
Eliminates / Reduces bacteria and other contaminates in food processing
Improves dairy herd milk production up to 28%
Improves insecticide effectiveness – Use less chemicals to achieve same results 5

5. Plasma Activated Water Technology
Agriculture
Eliminates bacteria and viruses on produce for a healthier food source
Studies have shown that fruits and vegetables washed with a low pH water will eliminate or reduce bacteria such as E.Coli, Pseudomonas, Staphylococcus, Salmonella and many others. 6

6. Plasma Activated Water Technology
Agriculture
Improves plant growth
Example: Cow Peas
The Plant on Left:
Was grown using High pH Water. The plant reached over twelve inches in height within one month.
The Plant on Right:
Was from the same seed lot, and given the same volume of ordinary tap water which grew to about one half the size of the other plant that was given High pH water in one month
Test Period Was For Approximately One Month Ending June 19, 2007 7

7. Plasma Activated Water Technology Sprayed With High pH Water
Agriculture
Improves plant growth
Eliminates bacteria and viruses in plants for a healthier food source
Extends fresh fruits and vegetables shelf life by 7 to 14 days.
This test was completed over a seven day period. All the strawberries were left exposed at room temperature.
The strawberry in the center was treated with High pH water, the others with tap water.
Sprayed With High pH Water
Not Treated
Not Treated 8
Test Was Completed Over a 7 Day Period in Open Air and at Room Temperature.

8. Plasma Activated Water Technology
Will be considered one of the most modern day marvels of this century.
Industrial Sterilization
Early test results have demonstrated that Hydro Enterprises low pH Plasma Activated Water can eliminate or reduce bacteria and viruses, including but not limited to Bacillus Subtilis, E Coli, Staphylococcus Aureus, Salmonella, Coliform MPN, Poliovirus, Pseudomonas, Rotavirus and MS2 Bacteriophage 9

9. Plasma Activated Water Technology
Will be considered one of the most modern day marvels of this century.
Manufacturing
Any manufacturing company that needs water with a controlled pH, sterilization requirement or monomolecular water structure can benefit greatly by utilizing Plasma Activated Water Technology in their manufacturing process. 10

10. Plasma Activated Water Technology
How Does This Work ? #1
This RF plasma process is unique because it is a non-chemical method of treatment. The RF plasma technology breaks the clusters of molecules in the water producing monomolecular water. The process uses a gas mixture of oxidizing and/or reducing agents that are ionized creating plasma inside of a quartz vessel. This newly created plasma contains ionization properties including charged particles, ultrasound modulation and ultra-violet radiation.   11

11. Plasma Activated Water Technology
How Does This Work ? #2
  The water source is then processed through the RF plasma reactor. The water crosses an electric/magnetic field and is also exposed to ultrasound and ultraviolet radiation from the RF plasma. This treatment destroys the molecular clusters contained in the water. The number of molecules in water clusters decrease from to 1-3. Water activity increases in proportion to the decrease of water clusters.   The monomolecular/activated water now has small sized water molecules allowing it to penetrate the membranes and pores of animals, plants and processed food. 12

12. Plasma Activated Water Technology
How Does This Work ? #3
Combined with a lower pH level, the monomolecular water becomes even a more powerful anti-bacterial agent making the water more acidic. What makes monomolecular low pH water one of the most effective and safe anti-bacterial tools on the market today is that this technology produces low pH water that is not harmful to humans, animals or plants but yet this water is one of the most effective anti bacteria products on earth. Even with the monomolecular water being at a low pH it will not burn human skin and can be disposed of without special handling.  13

13. How Water Is Effected With Plasma Activated Water Technology
H2O- molecule
WATER
How Water Is Effected With Plasma Activated Water Technology H
H2O O H
H2O
H2O
H2O
pH = 7 – 8
ORP = +500 mV
Size: 10 to 24 mol.
H2O
H2O
H2O
H2O
H2O
H2O
H2O
ORP – (e –activity)
Ph –Hydrogen index
(Concentration ratio H3O+ & (OH)-)
PLASMA
DC-Field
Na+
Cl-
H2O
H2O
H2O
(OH)- H+
H (OH)- K+
HCO3-
H2O
Ca+
H2O
H2O
(OH)- H+
H (OH)-
Mg2+
H2O
H2O
H2O
H2O
(OH)- predominant
pH : mV
pH: mV
H+ - predominant 14

14. What Happens As Water Passes Through The Plasma Activated Water System
RF Power Supply
Basic Frequency: 0.44 MHz – MHz
Modulation Frequency: 10 – 35 kHz
RF Pulse: kV
Pulse width: 5 – 45 microseconds
Gas System
(option)
Water Flow
Plasma
Gas Bubble UV
(<200 nm)
Blown Gas
Bubble
Ozone
Water/Liquid
Shock waves/
Ultrasound
E-field
What Happens As Water Passes Through The Plasma Activated Water System 15

15. Plasma Activated Water Technology
PAW Technology provides for the ability to raise or lower the pH of water to meet your needs. As water passes through the Radio Frequency Plasma Reactor, the water can be split into two types of water, each having their own benefits. One being a high pH activated water and the other a low pH activated water.
High pH Water Output
Source Water Input
Low pH Water Output
Examples of Benefits of High & Low pH Water
Improves health of humans, animals and plants Increases plant and animal production Reduce or eliminate NO2, NO3, CL, etc.
High pH
Low pH
Sanitizer/anti-bacterial/powerful disinfectant No added chemicals like CL or TSP Kills bacteria and viruses. 16

16. LOW TEMPERATURE PLASMA
ISOTHERMICAL PLASMA
NON-ISOTHERMICAL PLASMA
Arc Discharge
RF Discharge
Glow Discharge
Corona Discharge
Microwave Discharge
RF Discharge
Low Pressure
Te ~ Ti ~ Tg ~= 10,000 K
K = K0 exp(- Wakt./kT)
A2/(1-A2) x p = 3.32 x 10-2 x T5/2exp(-Wion/kT)
Te >> Ti ~ Tg
Te = 30,000 K
Ti ~ Tg ~ 375K 17

17. Water Properties 19 Parameter EPA Method Detection Limit Tap WaterpH
Conductivity
T. Dissolved Solids
TDS (summation)
MBAS
Color
Odor
Turbidity
Hardness
EPA Method
150.1
120.1
160.1
…….
425.1
110.1
140.1
180.1
130.2
Detection Limit
0.1 units
0.1 hOm/cm
5 mg/l
…mg/l
0.05 mg/l
0.5 unit
1.0 TON
0.5 NTU
0.5 mg/l
Tap Water
8.3
244
388
391
0.07 14
<1.0
<0.5
189
Activated Water
2.7
330
378
408
0.10 10
133
Calcium
Magnesium
Sodium
Potassium
Cations
Copper
Iron
Manganese
200.7
200.8
0.02 mg/l
0.1 mg/l
…meq/l
0.01 mg/l
0.03 mg/l 42 17 53
3.4
5.9
<0.01
0.09 27 15 84
1.6
6.3
0.01
0.06 19

18. Plasma Activated Water Treatment System
General Water, Disinfection and Sterilization Uses
RF/DC
Power Supply B
C-1
LP /drain
By-product(20%)
HP product (80%)
Key
A.) Water is supplied
B.) Passes through the plasma activated water treatment system
C.) Exits to either the low pH (C-1) or high pH (C-2) outlets
pH/ORP
Filter 1
pH: p
Water Source G A 2
Self
Cleaning
System
(Auto/Man)
Water Holding
Tank
C-2
IDEC (micro SMART)
To Application
Pump
5000 GPH
NOTE:
The holding tank can be used by either low, high pH output or both depending on the volume and requirements needed. 20

19. Will be considered one of the most modern day marvels of this century.
Plasma Technology
Will be considered one of the most modern day marvels of this century.
Wastewater Treatment
With the benefits of eliminating or greatly reducing bacteria, viruses and other harmful contaminants in water, this purification system could be used in many water treatment applications. With capabilities of purifying water at a rate of up to 100,000 gallons per hour, this technology offers a very cost effective means of wastewater treatment. 21

20. Anti-bacterial treatment, pH Control
TYPICAL WASTE WATER TREATMENT SYSTEM
INCOMING
SEWAGE
ENERGY RECOVERY
HEADWORKS
SEDIMENTATION
Methane
Capture sand and
solids
Bar Screens:
Remove wood,
Plastics and rags
PRIMARY TREATMENT
DIGESTER
Sludge
coagulation
PLASMA SYSTEM
Anti-bacterial treatment, pH Control
Option: Reverse Osmosis or Filtration System
Treated Water
De-Watering
Re-used Bio-solids
Clear Water
Water Re-Use
Option: CHLORINATION
IF REQUESTED 22

21. MICROBIOLOGY LABORATORY, ENVIRONMENTAL MONITORING DIVISION WASTEWATER 5 MILE EFFLUENT
Sample Date: 30-Jul-03
Analysis Date: 07/31/03
Total Coli-form
Fecal Coli-form
Entero
Read by
CES
AMA
Time
13:25
14:40
Before Treatment, CFU/100ml
2,400,000
4,800,000
2,000
After Treatment, CFU/100ml
< 1
Southern California Laboratory – SILLIKER , Inc.
Sample Date: 30-Jul-03
Analysis Date: 08/01/03
SCA
Coli-Forms
Heterotrophic Plate Count
Note
Water Before Treatment
> 1,600,000 / g
34,000 / ml
5 tube MPN
Water After Treatment
< 2 / g
60 / ml
Sludge Before Treatment
170,000 / g
Sludge After Treatment
17,000 / g 23

22. Sterilization PAW Is Effective Against: 24
Sterilization by steam or dry heat is not useful to sterilize materials that are adversely affected by such heat or steam
Ethylene Oxide E2H4O gas: suffer from residues on the articles to be sterilized, which may have adverse effects
Radiation Sterilization: limited to large industrial applications because of the large investment to build the gamma-radiation facilities, safe operation and change of bulk properties of non-metallic materials, especially food.
Plasma Sterilization: require special equipment (vacuum chamber, pumps, gas) and qualified operator.
Plasma activated water sterilization: no chemicals; safe; disposable; simple.
PAW Is Effective Against:
Viruses and bacterial Spores: Bacillus Subtilis, E Coli, Staphylococcus Aureus, Salmonella, Coliform MPN, Poliovirus, Pseudomonas, Rotavirus and MS2 Bacteriophage
Complex structures such as multilayer Albumen Coats
Dental bacteria such as Polysaccharide Exopolymers and Bio-films
PAW Is Effective With:
Species with high sporicidal conyesounds such as OH,H,O
Highly reactive components such as H2 O2, CLO, HOCL, CL, H+, etc 24

23. Spore Test Strip Test Table 1. BG Spore Strip Test Summary
Solution
Number of Spray Passes
Hydro Enterprises provided activated water.
This number represents the amount of decrease in spores present. The term “BG spores” refers to Bacillus Subtilis, which is often used as a surrogate for Anthrax. 2 6 10
PAW
3 – 6log
>6 log
Sprayer (minimized fluid consumption)
Nozzle orientation:
moved along the surface at a speed of 1 feet/sec and 2 feet from the surface
Validation: sampling with swabs, cultured. 19 25

24. Test Results 26 Fluid Decontaminant Sporalized Bacteria
Bacillus anthracis
(Ba)
Sporalized Bacteria Simulant
Bacillus subtilis var globigii (BG)
Viral Simulant
MS2
Product Comments
Easy DECON 
>6 log
>4 log
The MSDS for this solution should be read thoroughly before use. It is less caustic than most other solutions. This solution is produced in the United States and is available through EnviroFoam.
Hydrogen Peroxide 3%
Use carefully and read the MSDS thoroughly before use. The easy availability and handling of this fluid make it very appealing as a solution. The solution has a nearly unlimited shelf-life.
Hypochlorous Acid (0.5%)
>7 log
Use carefully and read the MSDS thoroughly before use. The easy availability of the foundation for this fluid, bleach, makes it appealing as a solution but the method to convert simple bleach into a properly mixed hypochlorous acid solution is somewhat complex. Solution has a limited shelf-life, but it should be used within two-three hours after preparation.
Antibacterial Water
N/A
Contact Hydro Enterprises for further information. 26

25. Each Sample Represent 30 Second Shake With Either Low pH Water, High pH Water or TSP Solution In Turkey Processing Plant
CFU's
MESO
PHSYC
ECOLI
TCOL
STAPH YM
Lactics
SAL
LIST
LO pH
3,929
2,821 43 67 57 36 79 1
HI pH
6,586
3,671 39 80
147 53 83
BUFFER
17,314
9,900
1,333
1,719 91
101
360 4
TSP/BUF
9,400
8,271
383
533
149 66
141 2 27

26. ACTIVATED H20 TEST 28 Test MESO PHSYC ECOLI TCOL LACTICS SAL Egg Floor
< 10
Neg.
Egg - Nest
Final LP water
Not tested
Final HP Water
370
490 30 20 28

27. SANITATION Sausage Manufacture The natural casings: experimental data
Results:
1. Casing Sample out of the barrel………… ,000 col/g
2. Casing Sample just rinsed with tap water…180,000 col/g
3. Casing treated with Activated water -L ,500 col/g
4. Casing treated with Activated water -L ,600 col/g
Notes:
1.Treatment time = 45 sec
2. Analyzed for aerobic plate count
3. The use of the activated water resulted in a 2 log reduction
of the bacteria load on the casings 29

28. Plasma Activated Water Technology
Why Is It safe ?
Through the process the pH and ORP of water is increased or decreased to meet your needs, along with eliminating or reducing bacteria, viruses and other contaminants. The low pH water does not have the typical burn effects found with fluids at very low pH levels. This patented technology coupled with industry standard filtration systems produce clean Plasma Activated Water for your needs. 30

29. Competitive Technology Used To Create Activated Water
Electro-Chemical
Magnetic Field
Freeze and Thaw
Action of Acoustic Oscillation
Heat Impact under the Pressure
Our Plasma Activated Water Technology is the most versatile, cost effective and reliable system available in the world today. 31

30. Hydro Enterprises, Inc.
Contact for Business Development Inquiries:
Burton Danet, Ph.D.
abc4all (at) gmail (dot) com
Water Treatment Equipment available from 100 gallons per hour up to 100,000 gallons per hour. 32

31. Water Plasma Activated Water Technology Life’s Most Precious Resource
Health
Manufacturing
Medical Sterilization
Agriculture
Bio Chemical Sterilization
Industrial Sterilization
Waste Water Treatment
General Water Improvements
Bottled Water
Emergency Water Treatment