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Published byJohan Budiaman Modified over 6 years ago
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Role of C-terminal phosphorylation in the subcellular localization of AtPIP2;1.A, the figure shows typical laser-scanning confocal micrographs of the fluorescence emitted by root epidermal cells at 1 cm from the apex in transgenic plants that express GFP-PIP2;1, GFP-PIP2;1-S280A, GFP-PIP2;1-S283A, or GFP-PIP2;1-S283D. Role of C-terminal phosphorylation in the subcellular localization of AtPIP2;1.A, the figure shows typical laser-scanning confocal micrographs of the fluorescence emitted by root epidermal cells at 1 cm from the apex in transgenic plants that express GFP-PIP2;1, GFP-PIP2;1-S280A, GFP-PIP2;1-S283A, or GFP-PIP2;1-S283D. Scale bar, 20 μm. B, the graph represents the proportion of root cells at 1 cm from the apex with an intracellular staining. Data were obtained from transgenic plants that express the following constructs: GFP-PIP2;1 (n = 13 plants from two independent transgenic lines), GFP-PIP2;1-S280A (n = 9 plants from three independent transgenic lines), GFP-PIP2;1-S283A (n = 7 plants from two independent transgenic lines), and GFP-PIP2;1-S283D (n = 6 plants from two independent transgenic lines). The numbers on the graph correspond to the total number of observed cells. The error bars represent S.D. Sodana Prak et al. Mol Cell Proteomics 2008;7: © 2008 The American Society for Biochemistry and Molecular Biology
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