1. ChemiSTEM data derived from the region immunoreactively binding nanogold particles and needle-shaped structures of the Eoconfuciusornis feathers (sample 205), and compared with feathers of G. gallus.
ChemiSTEM data derived from the region immunoreactively binding nanogold particles and needle-shaped structures of the Eoconfuciusornis feathers (sample 205), and compared with feathers of G. gallus. (A) HADDF image of an immunogold-labeled section of the Eoconfuciusornis feather, indicating the region selected for elemental mapping. (B–D) Various element distributions for the immunogold-labeled region (arrowhead). (B) Gold (Au) distribution map indicating the localization of nanoparticles. (C) Sulfur map showing the distribution of this element of overlap with antibody-bound Au beads. (D) Nitrogen map suggesting the entire tissue is dominated by N, consistent with a proteinaceous source. (F–H) Element distributions for the needle-shaped structures observed in some regions of the fossil feather. (F) Calcium distribution outlines the shape of the structure, indicating it contains a relatively high Ca level. (G and H) Nitrogen and phosphorus distribution maps, respectively, which are not specifically localized within the needle-shaped structures. (E) HADDF image of an immunogold-labeled section of G. gallus feather, indicating the region selected for elemental mapping. (I–K) Various element distributions for the immunogold-labeled region. (I) Au distribution map. (J) S map. (K) N map. (Scales are as indicated.)‏
Yanhong Pan et al. PNAS 2016;113:49:E7900-E7907
©2016 by National Academy of Sciences