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Quantification of C-terminal phosphorylation of AtPIP2;1 upon NaCl (A) and H2O2 (B) treatments.A, plants were either untreated (white bars) or treated with 100 mm NaCl for 2 h (gray bars) or 4 h (black bars). Quantification of C-terminal phosphorylation of AtPIP2;1 upon NaCl (A) and H2O2 (B) treatments.A, plants were either untreated (white bars) or treated with 100 mm NaCl for 2 h (gray bars) or 4 h (black bars). The proportion of the unmodified (0P), singly phosphorylated on Ser280 (1P), and diphosphorylated on Ser280 and Ser283 (2P) peptides was quantified in plant extracts as exemplified in Fig. 5. Letters a–d indicate statistically different values (Mann and Whitney, p < 0.05). B, plants were untreated (white) or treated with 2 mm H2O2 for 15 min (black). The same procedures and conventions as in A were used. Sodana Prak et al. Mol Cell Proteomics 2008;7: © 2008 The American Society for Biochemistry and Molecular Biology
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