Presentation is loading. Please wait.

Presentation is loading. Please wait.

A B C D CD4+ CD8+ Control Isotype Ab Anti-TNFa Ab Control Isotype Ab

Published byEthan Hines Modified over 6 years ago

Similar presentations

Presentation on theme: "A B C D CD4+ CD8+ Control Isotype Ab Anti-TNFa Ab Control Isotype Ab"— Presentation transcript:

1 A B C D CD4+ CD8+ Control Isotype Ab Anti-TNFa Ab Control Isotype Ab Anti-TNFa Ab E F G H Supplementary Figure 2. Flow cytometric gating strategies for intracellular cytokine analysis. Lung cells isolated at day 10 post infection were stimulated for five hours in vitro with PMA and Ionomycin prior to staining for flow cytometric analysis. Lmphocytes were gated (A) and the doublets removed using a pulse width gate (B). Dead cells were removed using a Live/Dead gate (C) before gating the CD4+ and CD8+ lymphocyte populations (D). Cytokine expression by both CD4+ (E & F) and CD8+ (G & H) T cells was determined by the use of isotype control antibodies. IFNg secretion by CD4+ and CD8+ T cells was also determined using this gating strategy.

Download ppt "A B C D CD4+ CD8+ Control Isotype Ab Anti-TNFa Ab Control Isotype Ab"

Similar presentations

A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.

A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.
Early HIV cohort: Frozen PBMC samples from all 37 HIV-1 infected subjects; (77% of subjects within 6 months of seroconversion) (HIV negative or control.

Early HIV cohort: Frozen PBMC samples from all 37 HIV-1 infected subjects; (77% of subjects within 6 months of seroconversion) (HIV negative or control.
Isotype control Supplementary Figure S1. Strategy for gating CD11b+CD14-CD33+CD40+ myeloid cells. Singlets were gated from live cells shown in forward.

Isotype control Supplementary Figure S1. Strategy for gating CD11b+CD14-CD33+CD40+ myeloid cells. Singlets were gated from live cells shown in forward.
IFNγIL-2TNFaIL-17A CD40 L MOPP DMSO CD4+ Supplemental figure 5A SEB isotype control isotype control isotype control CD69+ A.

IFNγIL-2TNFaIL-17A CD40 L MOPP DMSO CD4+ Supplemental figure 5A SEB isotype control isotype control isotype control CD69+ A.
Volume 125, Issue 2, Pages (August 2003)

Volume 125, Issue 2, Pages (August 2003)
Figure 1. Overexpression of PD-1 decoy increases IFN-γ secretion from T cells. Activated B6 splenic T cells were transduced with retrovirus carrying either.

Figure 1. Overexpression of PD-1 decoy increases IFN-γ secretion from T cells. Activated B6 splenic T cells were transduced with retrovirus carrying either.
Foxp3 induction dependent on cell cycle progression in vitro.

Foxp3 induction dependent on cell cycle progression in vitro.
Memory B cells IgM only Marginal zone B cells without plasmablasts

Memory B cells IgM only Marginal zone B cells without plasmablasts
Supplementary Figure S1: The secondary structure of C1q is irreversibly altered after heat-denaturation. The far UV spectrum was measured by circular dichroism.

Supplementary Figure S1: The secondary structure of C1q is irreversibly altered after heat-denaturation. The far UV spectrum was measured by circular dichroism.
A B C Supplementary Figure 1 Group 1 Group 2 Group 3 #1 #2 #3 #4 #5 #6

A B C Supplementary Figure 1 Group 1 Group 2 Group 3 #1 #2 #3 #4 #5 #6
Volume 140, Issue 1, Pages e2 (January 2011)

Volume 140, Issue 1, Pages e2 (January 2011)
Volume 141, Issue 1, Pages e2 (July 2011)

Volume 141, Issue 1, Pages e2 (July 2011)
by Jason M. Brenchley, Mirko Paiardini, Kenneth S. Knox, Ava I

by Jason M. Brenchley, Mirko Paiardini, Kenneth S. Knox, Ava I
Volume 136, Issue 3, Pages (March 2009)

Volume 136, Issue 3, Pages (March 2009)
Wei Hu, Ty Dale Troutman, Ramakrishna Edukulla, Chandrashekhar Pasare 

Wei Hu, Ty Dale Troutman, Ramakrishna Edukulla, Chandrashekhar Pasare 
Lung Natural Helper Cells Are a Critical Source of Th2 Cell-Type Cytokines in Protease Allergen-Induced Airway Inflammation  Timotheus Y.F. Halim, Ramona H.

Lung Natural Helper Cells Are a Critical Source of Th2 Cell-Type Cytokines in Protease Allergen-Induced Airway Inflammation  Timotheus Y.F. Halim, Ramona H.
Preformed CD40 ligand exists in secretory lysosomes in effector and memory CD4+ T cells and is quickly expressed on the cell surface in an antigen-specific.

Preformed CD40 ligand exists in secretory lysosomes in effector and memory CD4+ T cells and is quickly expressed on the cell surface in an antigen-specific.
Skin-Resident Effector Memory CD8+CD28– T Cells Exhibit a Profibrotic Phenotype in Patients with Systemic Sclerosis  Gang Li, Adriana T. Larregina, Robyn.

Skin-Resident Effector Memory CD8+CD28– T Cells Exhibit a Profibrotic Phenotype in Patients with Systemic Sclerosis  Gang Li, Adriana T. Larregina, Robyn.
A B C D E F Supplementary Fig. 1. Gating strategy for CD44+CD133+CD24+ B16 tumor initiating cells (TIC). Cells were gated on CD44. CD44 positive cells.

A B C D E F Supplementary Fig. 1. Gating strategy for CD44+CD133+CD24+ B16 tumor initiating cells (TIC). Cells were gated on CD44. CD44 positive cells.
A B C D E F Supplementary Fig. 2. Gating strategy for CD44+CD24+ ID8agg tumor initiating cells (TIC). Cells were gated on CD44. CD44 positive cells were.

A B C D E F Supplementary Fig. 2. Gating strategy for CD44+CD24+ ID8agg tumor initiating cells (TIC). Cells were gated on CD44. CD44 positive cells were.

Similar presentations

Ads by Google