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Published byDanielle Lheureux Modified over 6 years ago
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A B C Supplementary Figure 1 Group 1 Group 2 Group 3 #1 #2 #3 #4 #5 #6
# # #3 Group 1 # # #6 CD20 Group 2 C # # #9 Group 3 Her-2/neu MHC I
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Supplementary Figure 1. Confirmation of Her-2/neu expression on autologous B lymphoblastoid cells (BLCL) transduced with retroviral vector expressing Her-2/neu. A. Autologous BLCL and BLCL-HM were stained with PE-conjugated anti-Her-2/neu antibody. Binding of anti-Her-2/neu Ab was analyzed by flow cytometry. BLCL is shown as a filled area, and BLCL-HM is shown as a solid line. B. BLCL #1 was stained with FITC-conjugated anti-human CD20 antibody. CD20 expression is shown as a solid line. Binding of the control antibody is shown as a filled area. C. BLCL #1 was stained with PE-conjugated anti-human MHC I antibody. The level of MHC I is shown as a solid line. Binding of the control antibody is shown as a filled area. Data are representative of at least three separate experiments.
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