Presentation is loading. Please wait.

Presentation is loading. Please wait.

Modulation of basophil activity: A novel function of the neuropeptide α-melanocyte– stimulating hormone  Markus Böhm, MD, Mara Apel, Koji Sugawara, MD,

Published byΊρις Ευταξίας Modified over 5 years ago

Similar presentations

Presentation on theme: "Modulation of basophil activity: A novel function of the neuropeptide α-melanocyte– stimulating hormone  Markus Böhm, MD, Mara Apel, Koji Sugawara, MD,"— Presentation transcript:

1 Modulation of basophil activity: A novel function of the neuropeptide α-melanocyte– stimulating hormone  Markus Böhm, MD, Mara Apel, Koji Sugawara, MD, Randolf Brehler, MD, Kerstin Jurk, PhD, Thomas A. Luger, MD, Helmut Haas, PhD, Ralf Paus, MD, Britta Eiz-Vesper, MD, Andrew F. Walls, PhD, Evgeni Ponimaskin, PhD, Manuela Gehring, Alexander Kapp, MD, Ulrike Raap, MD  Journal of Allergy and Clinical Immunology  Volume 129, Issue 4, Pages (April 2012) DOI: /j.jaci Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Isolated human basophils (A-C) and tissue basophils (D) express MC-1Rs. Fig 1, A, RT-PCR of MC-Rs, positive controls (PC; cDNA from melanocytes or genomic DNA from fibroblasts), and negative controls (H2O or basophil mRNA without RT). MC-1R expression, as shown by using Western immunoblotting (Fig 1, B), FACS analysis (Fig 1, C), and MC-1R/BB1 double immunostaining of nasal tissue (Fig 1, D). Depicted are representative experimental sets of 3 donors. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 POMC expression in isolated human basophils. A, Detection of exon 3–related POMC transcripts by means of RT-PCR. B, RT-PCR of POMC transcripts derived from exons 2 and 3. Positive controls were cDNA from melanocytes or normal skin, and negative controls were H2O or basophil mRNA without reverse transcription (RT). C, POMC protein expression as shown by using Western immunoblotting. Depicted are representative experimental sets of 3 donors. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Effects of α-MSH on intracellular Ca2+ and cAMP levels in isolated normal human basophils. A and B, Ca2+ mobilization after α-MSH or C5a (10−8 mol/L) treatment was measured in a buffer containing 1 mmol/L (Fig 3, A) or 100 nmol/L CaCl2 (Fig 3, B). Depicted are representative experiments of 6 to 8 donors. C, cAMP levels after α-MSH or forskolin (1 μmol/L) treatment for 20 minutes (n = 4). ∗∗P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Effects of melanocortins in the BAT. A, No effect of α-MSH alone (n = 3). B, CD63 surface expression after fMLP stimulation. Suppression of this effect by α-MSH is shown (n = 7). ∗∗∗P < C, ACTH suppresses fMLP-induced CD63 expression (n = 6). ∗∗∗P < .001, ∗∗P < .01, and ∗P < .05. ACTH was given alone (10−6 mol/L) or with fMLP. α-MSH (10−6 mol/L) served as a control. D, Suppression of PMA-induced CD63 expression by α-MSH (n = 3). ∗P < .05 and ∗∗P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 The effect of α-MSH on basophil activation is MC-1R dependent. A, Preincubation with a peptide analogue of ASIP (10−7 mol/L) neutralizes the inhibitory effect of α-MSH (10−6 mol/L) on fMLP-induced CD63 expression in the BAT (n = 4). ∗∗P < .01 and ∗∗∗P < B, α-MSH, but not KPV and KdPT, suppresses fMLP-induced basophil activation in the BAT (n = 5). ∗∗∗P < .001. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig 6 α-MSH suppresses allergen-induced basophil activation, as shown in the BAT. Increased surface expression of CD63 after stimulation of basophils with anti-IgE or grass pollen (GP) in patients with allergic rhinitis to grass pollen is shown (n = 4). Dose-dependent suppression of grass pollen induced upregulation of CD63 after stimulation with α-MSH, whereas α-MSH (10−6 mol/L) per se displayed no effect (n = 4). ∗∗P < .01 and ∗P < .05. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig 7 A-C, IL-4, IL-6, and IL-13 release by isolated basophils from different donors (n = 11 for IL-6, n = 5 for IL-4, and n = 5 for IL-13) after α-MSH treatment (10−8 mol/L, 4 hours). D-F, Inhibition of IL-4 (Fig 7, D), IL-6 (Fig 7, E), and IL-13 (Fig 7, F) release by α-MSH preincubation (20 minutes) and subsequent anti-IgE, fMLP, or PMA stimulation (4 hours). Mann-Whitney rank sum test: ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Modulation of basophil activity: A novel function of the neuropeptide α-melanocyte– stimulating hormone  Markus Böhm, MD, Mara Apel, Koji Sugawara, MD,"

Similar presentations

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.)  Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.)  Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,
Dorothea Dijkstra, MSc, Rob Leurs, PhD, Paul Chazot, PhD, Fiona C

Dorothea Dijkstra, MSc, Rob Leurs, PhD, Paul Chazot, PhD, Fiona C
Early suppression of basophil activation during allergen-specific immunotherapy by histamine receptor 2  Natalija Novak, MD, Nihal Mete, MD, Caroline.

Early suppression of basophil activation during allergen-specific immunotherapy by histamine receptor 2  Natalija Novak, MD, Nihal Mete, MD, Caroline.
Pharmacologic rationale for treating allergic and nonallergic rhinitis

Pharmacologic rationale for treating allergic and nonallergic rhinitis
The functional activity of basophil granulocytes is modulated by acute mental stress and sympathetic activation in vivo and in vitro  Ulrike Raap, MD,

The functional activity of basophil granulocytes is modulated by acute mental stress and sympathetic activation in vivo and in vitro  Ulrike Raap, MD,
High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,

High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,
Margaretha A. Faber, MD, Vito Sabato, MD, Chris H

Margaretha A. Faber, MD, Vito Sabato, MD, Chris H
Human circulating group 2 innate lymphoid cells can express CD154 and promote IgE production  Laura Maggi, PhD, Gianni Montaini, BSc, Alessio Mazzoni,

Human circulating group 2 innate lymphoid cells can express CD154 and promote IgE production  Laura Maggi, PhD, Gianni Montaini, BSc, Alessio Mazzoni,
Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response  Miriam Wittmann, MD, Mareike Alter, Tanja.

Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response  Miriam Wittmann, MD, Mareike Alter, Tanja.
Ibrutinib, a Bruton’s tyrosine kinase inhibitor used for treatment of lymphoproliferative disorders, eliminates both aeroallergen skin test and basophil.

Ibrutinib, a Bruton’s tyrosine kinase inhibitor used for treatment of lymphoproliferative disorders, eliminates both aeroallergen skin test and basophil.
Antigen-driven basophil activation is indicative of early Necator americanus infection in IgE-seronegative patients  Franco H. Falcone, PhD, Gary Telford,

Antigen-driven basophil activation is indicative of early Necator americanus infection in IgE-seronegative patients  Franco H. Falcone, PhD, Gary Telford,
Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 
Protease-activated receptor 2–dependent fluid secretion from airway submucosal glands by house dust mite extract  Hyung-Ju Cho, MD, PhD, Hyun Jae Lee,

Protease-activated receptor 2–dependent fluid secretion from airway submucosal glands by house dust mite extract  Hyung-Ju Cho, MD, PhD, Hyun Jae Lee,
Prostaglandin E2 suppresses CCL27 production through EP2 and EP3 receptors in human keratinocytes  Naoko Kanda, MD, PhD, Hiroshi Mitsui, MD, PhD, Shinichi.

Prostaglandin E2 suppresses CCL27 production through EP2 and EP3 receptors in human keratinocytes  Naoko Kanda, MD, PhD, Hiroshi Mitsui, MD, PhD, Shinichi.
Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule  Carine Metz-Favre, MD, Birgit Linhart, PhD, Margarete Focke-Tejkl, PhD,

Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule  Carine Metz-Favre, MD, Birgit Linhart, PhD, Margarete Focke-Tejkl, PhD,
Histamine H2 receptor stimulation upregulates TH2 chemokine CCL17 production in human M2a macrophages  Susanne Mommert, PhD, Karl Gregor, MD, Kristine.

Histamine H2 receptor stimulation upregulates TH2 chemokine CCL17 production in human M2a macrophages  Susanne Mommert, PhD, Karl Gregor, MD, Kristine.
Cyclosporin H is a potent and selective competitive antagonist of human basophil activation by N-formyl-methionyl-leucyl-phenylalanine  Amato de Paulis,

Cyclosporin H is a potent and selective competitive antagonist of human basophil activation by N-formyl-methionyl-leucyl-phenylalanine  Amato de Paulis,
Eniko Sonkoly, MD, Anja Muller, MD, Antti I

Eniko Sonkoly, MD, Anja Muller, MD, Antti I
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
Suzana Radulovic, MD, Mikila R. Jacobson, PhD, Stephen R

Suzana Radulovic, MD, Mikila R. Jacobson, PhD, Stephen R

Similar presentations

Ads by Google