1. Embryologic outcome and secretome profile of implanted blastocysts obtained after coculture in human endometrial epithelial cells versus the sequential system 
Francisco Dominguez, Ph.D., Blanca Gadea, Ph.D., Amparo Mercader, Ph.D., Francisco J. Esteban, Ph.D., Antonio Pellicer, M.D., Ph.D., Carlos Simón, M.D., Ph.D. 
Fertility and Sterility 
Volume 93, Issue 3, Pages e1 (February 2010)
DOI: /j.fertnstert
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Protein array membranes showing the protein abundance in different culture situations. Each spot pair corresponds to one studied protein. Protein spot intensity correlates with protein abundance in the analyzed medium. Clear differences are shown in some proteins (arrows and circles). (A) Protein profile of the endometrial epithelial cell (EEC) coculture conditioned media. (B) Protein profile of the sequential conditioned media.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Protein quantification of proteins from the endometrial epithelial cell (EEC) coculture and sequential systems. The relative mean expression of each protein studied is shown in the EEC coculture conditioned media (blue bars) and the sequential medium (red bars). The densitometry values were transformed into the logarithmic scale (log2) and normalized using the quantile normalization function, as implemented in the Bioconductor Limma package. Data are expressed in log arbitrary densitometry units ± SD. ∗Statistical difference in the relative expression mean (P<.05) between the EEC coculture and sequential systems.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
(A) Comparison of IL-6 abundance in the conditioned medium from the endometrial epithelial cell coculture and the control medium (CCM) using ELISA. Data are expressed in pg/μL ± SE. ∗∗Statistical difference compared with the control (P<.01). (B) Comparison of IL-6 difference in conditioned medium from implanted and nonimplanted CCM sequential conditioned media. In this case, data were obtained by subtracting the control medium from every conditioned medium studied from each embryo analyzed. Data are expressed in pg/μL ± SE. ∗∗Statistical difference compared with the control (P<.01).
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Ingenuity network analysis constructed using the three significant (false discovery rate <0.05) and differentially detected proteins in the conditioned media from endometrial epithelial cell coculture versus sequential culture as seed molecules. The top Ingenuity associated network functions were Cellular Development, Cellular Growth and Proliferation, and Connective Tissue Development and Function. 12(S)-HPETE = (12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoic acid; G22RIK = RIKEN cDNA G22 gene; AF = cDNA sequence AF251705; BTNL2 = butyrophilin-like 2 (major histocompatibility complex class II associated); CARD9 = caspase recruitment domain family, member 9; CD6 = CD6 molecule; CHST4 = carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; CIB2 = calcium and integrin–binding family member 2; CLEC4E = C-type lectin domain family 4, member E; CLEC7A = C-type lectin domain family 7, member A; CRLF1 = cytokine receptor–like factor 1; CX3CR1 = chemokine (C-X3-C motif) receptor 1; CXCL13 = chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); FCGR1B = Fc fragment of IgG, high affinity Ib, receptor (CD64); FCGR1C = Fc fragment of IgG, high affinity Ic, receptor (CD64); FCGRT = Fc fragment of IgG, receptor, transporter, alpha; FGF2 = fibroblast growth factor 2 (basic); GBP1 (includes EG:14468) = guanylate nucleotide binding protein 1; GPNMB = glycoprotein (transmembrane) NMB; IL19 = interleukin-19; IL1F6 = interleukin-1 family, member 6 (epsilon); IL1F9 = interleukin-1 family, member 9; IL6 = interleukin-6 (interferon, beta 2); KCTD13 = potassium-channel tetramerization domain containing 13; MT1E = metallothionein-1E; NFKBIZ = nuclear factor of kappa light polypeptide gene enhancer in B-cell inhibitor, zeta; RETNLB (includes EG:57263) = resistin-like beta; SLCO1A1 = solute carrier organic anion transporter family, member 1a1; STAP2 = signal transducing adaptor family member 2; TLR6 = toll-like receptor 6; TNF = tumor necrosis factor (TNF superfamily, member 2); TNIP3 = TNFAIP3-interacting protein 3; TREM1 = triggering receptor expressed on myeloid cells 1; UBR2 = ubiquitin protein ligase E3 component n-recognin 2; IL6R = interleukin-6 receptor; LTA = lymphotoxin alpha (TNF superfamily, member 1); IL19 PREDICTED = interleukin-19 (predicted).
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions