1. 2GxABT, ULg, Passage des Déportés, 2. B-5030 Gembloux, Belgium
Effects of maternal wheat bran supplementation on microbiota and intestinal parameters of piglets
J. Leblois1,2, S. Massart2, J. Wavreille3, B. Li2, J. Bindelle2, N. Everaert2
1 FRIA-FNRS, Fonds de la Recherche Scientifique, Rue d’Egmont, Brussels, Belgium
2GxABT, ULg, Passage des Déportés, 2. B-5030 Gembloux, Belgium
3Walloon Agricultural Research Centre, Rue de Liroux, 9. B-5030 Gembloux, Belgium

2. Use and abuse of antibiotics (AB)
Introduction
Objective
Methods
Results
Conclusion
Weaning piglets
Mutiple stresses:
Nutritional
Emotionnal
Social
Gut disorders
ETEC infections
Less absorption of nutrients
Use and abuse of antibiotics (AB)

3. Introduction
Objective
Methods
Results
Conclusion

4. Use and abuse of antibiotics
Introduction
Objective
Methods
Results
Conclusion
Use and abuse of antibiotics
Multi-resistant bacteria
Probiotics
Need to find sustainable alternatives
Zinc oxide
Prebiotics
Act on microbiota of the piglets

5. Use and abuse of antibiotics
Introduction
Objective
Methods
Results
Conclusion
Use and abuse of antibiotics
Multi-resistant bacteria
Need to find sustainable alternatives
Act on microbiota of the piglets
Maturation of gut immune system
Production of beneficial SCFA

6. Timing? Piglets before and/or at weaning Sows  before birth
Introduction
Objective
Methods
Results
Conclusion
Timing?
Piglets before and/or at weaning
Sows  before birth
Microbiota transfer
Faeces during lactation
Milk
Umbilical cord

7. Introduction Objective Methods Results Conclusion Wheat bran
Intestinal inflammatory response
Microbiota
SCFA production
Villus height/crypt depth

8. Weaning: 16 female piglets euthanized
Introduction
Objective
Methods
Results
Conclusion
7 CON sows & 8 WB sows
Gestation 240g/kg DM WB
Lactation 140g/kg DM WB
Weaning: 16 female piglets euthanized

9. Introduction Objective Methods Results Conclusion Colon content
Illumina MiSeq
Duodenum, jejunum, ileum
Haematoxylin/eosin coloration
Ileum, caecum, colon contents
SCFA

10. Ileum tissue Incubation 2 hours, 39°C, 5% CO2 Introduction Objective
Methods
Results
Conclusion
Ileum tissue
TLR4
TNF-α
Control
LPS 10µg/ml
ZO-1
CLDN2
2 reference genes:
GAPDH
PPIA
Incubation 2 hours, 39°C, 5% CO2

11. Introduction Objective Methods Results Conclusion
5 genera differed between maternal treatment for piglets, including butyrate-producing Colinsella
Leblois, J. et al. (2017) Modulation of piglets ’ microbiota : differential effects by a high wheat bran maternal diet during gestation and lactation. Sci. Rep. 7, article 7426

12. Introduction Objective Methods Results Conclusion Ileum Caecum Colon
Acetate /
WB > CON
Butyrate
WB < CON
Valerate
* p<0.05
+ p<0.10

13. No impact of the LPS treatment
Introduction
Objective
Methods
Results
Conclusion
No impact of the LPS treatment
Due to dose, incubation time or E. coli strain?
No impact of the maternal treatment, considering or not LPS treatment

14. Introduction Objective Methods Results Conclusion
Maternal treatment effect?
Impact
Microbiota
Yes
5 genera impact, including butyrate-producing Colinsella. Transfer probably during lactation and gestation
SCFA
Less valerate WB pigs
Higher acetate WB pigs
Higher butyrate CON pigs
Histomorphology
Higher villi duodeneum WB pigs
Shorter crypts jejunum WB pigs
Higher ratio duodenum & jejunum WB pigs
Inflammatory response No
No impact of LPS challenge
No impact of maternal treatment

15. For the future: Metabolomics/metatranscriptomics for piglets?
Introduction
Objective
Methods
Results
Conclusion
For the future:
Metabolomics/metatranscriptomics for piglets?
Jejunum: quantify the expression of carbohydrate transporter to determine wether the higher ratio could impact nutrient absorption
Ex vivo: dose and incubation time may not be adequate

16. Acknowledgments National Fund For Scientific Research (FNRS-FRIA)
For financial support:
National Fund For Scientific Research (FNRS-FRIA)
University of Liège
For providing animals and facilities:
CRA-w