1. Biodiverisity of culturable endophytic fungi isolated from 4 mangrove species
Lirazan, Lauren Anais M.
Division of Biological Sciences
College of Arts and Sciences
UP Visayas, Miagao, Iloilo

2. INTRODUCTION

3. Background of the Study
“endophyte” (1866) by De Barry
any organism living within a plant that infects it without causing observable symptoms of disease (Humeera et al., 2015)
Fungi
plant-like organisms without chlorophyll
Background of the Study

4. Symbiotic relationship between host plant and fungi
Endophyte + Fungi
Symbiotic relationship between host plant and fungi
Symbiosis is the living together of dissimilar organisms

5. BACKGROUND OF THE STUDY
Endophytes have been found in all plants studied for their presence.
Endophytic fungi may be diverse on a small scale (Stone et al., 2004).

6. STATEMENT OF THE PROBLEM
more species remain unscreened for their endophytic communities
studies regarding the endophyte biodiversity of Philippine plant species are rare
absence and deficiency of basic taxonomic information is a major obstacle to ecological studies of endophytic fungi

7. OBJECTIVES
General
study the endophyte assemblages from leaves of four mangrove species along Iloilo River

8. OBJECTIVES
Specific
Isolate the endophytic fungi present in the selected mangrove species
Determine the species richness of endophytic fungi of the targeted mangrove species
Identify the dominant endophytic fungal taxa

9. Significance of the Study
Studies on endophytic fungi are expected to harbour species that have not been identified in the past.
Significance of the Study

10. Significance of the Study
Fungal endophytes may be the source of secondary metabolites and bioactive compounds
Significance of the Study

11. of prescription drugs are based on secondary metabolites
40%
of prescription drugs are based on secondary metabolites

12. Significance of the Study
Penicillium notatum (1928) by Sir Alexander Fleming
taxol from Taxomyces andreanae
Significance of the Study

13. Significance of the Study
Many plant species remain unexamined for their endophyte assemblages and the potential of these fungi remain untapped
Significance of the Study

14. SCOPE AND LIMITATIONS Precise periodic sampling will not be conducted
Isolation procedures allow that only fungal species that can survive on PDA will be isolated
Identification of isolates will be morphological alone and further characterization of isolated cannot be conducted

15. METHODOLOGY

16. Iloilo River
Estuary
From Oton to Iloilo Straight

17. Iloilo River
Estuary
From Oton to Iloilo Straight

18. PROPOSED PROCEDURE Four mangrove species were selected:
Avicennia rumphiana
Acanthus ebracteatus
Acanthus volublis
Sycphiphora hydrophyllacea

19. PROPOSED PROCEDURE Sample Processing and Sterilization Sterilization
Three healthy leaves per species
Three segments per leaf
Sterilization
submersion in distilled water for 1 minute, Zonrox for 2 minutes then allowed to dry

20. PROPOSED PROCEDURE
Potato Dextrose Agar (PDA) + Antibacterial Penicillin
Incubation
25°C and checked occasionally for significant growth from the leaf segment

21. Data Analysis

22. Jaccard Similarty Coefficient
𝐽= 𝐶 𝐴+𝐵−𝐶
Jaccard Similarty Coefficient
where A and B are the total number of fungal species isolated from any two hosts and C the number of fungal species found in common
Similarity between data sets
(Suryanarayanan and Kumaresan, 2000; Ventachatalam et al., 2014)

23. 𝐶𝑅= 𝑛𝑢𝑚𝑏𝑒𝑟 𝑜𝑓 𝑖𝑠𝑜𝑙𝑎𝑡𝑒𝑠 # 𝑜𝑓 𝑠𝑒𝑔𝑚𝑒𝑛𝑡𝑠 𝑦𝑖𝑒𝑙𝑑𝑖𝑛𝑔 𝑓𝑢𝑛𝑔𝑖 ×100
Colonization
Rate
(Sunayana N, et al., 2014)

24. Margalef’s richness index
𝑀= 𝑆−1 ln⁡(𝑛)
Margalef’s richness index
evenness with which individuals are divided among the taxa present
(Suryanarayanan and Kumaresan, 2000)

25. REFERENCES
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28. Thank you