1. Semmelweis Medical University Budapest, Hungary
Dorothy Tang Class of 2017

2. Nanomechanics of Fibrin and Factor H
Examined the effects of different ionic strengths on fibrin networks, particularly varying concentrations of calcium
Factor H
Examined the inner structure of factor H by unfolding its globular domains through experimentation of different buffers and centrifugation forces

3. Department of Biophysics

4. Most Rewarding Aspect
Learned how to use the Cypher for atomic force microscopy (AFM) imaging
Shadowed other projects in the university
Gave me the chance to use a machine the Cypher and learn the concept behind it to make these beautiful AFM images
Also able to shadow other projects in the university – saw how diverse the other projects were and how other machines were used like the optical laser

5. Gel Electrophoresis of Fibrin Networks Over Time
Alpha Domains
Beta Domains
Gamma Domains
We could see over time how the fibrin networks were formed through gel electrophoresis – fibrin is made up of alpha, beta and gamma
Gamma domains disappearing over time since gamma links are being formed to form the fibrin network
fgn
3 min
30 min
2 hrs

6. Spurring On Research
Contributed my ideas on how best to elongate factor H domains
Raised questions for more analysis in the future
I helped to contribute ideas on how best to elongate factor H domains and we found that a particular buffer and higher centrifugation forces would best elongate factor H. I also raised questions for more analysis in the future since there are much more to analyze

7. Future Plans
Working in the Biophysics Department opened my eyes to another world of biology
A more theoretical type of research
Continuing path of molecular biology
Possibly pursue more research in the future?
Made me more open to doing more research in the future

8. My mentor, Timi
Another rewarding aspect was making friends at the university, especially with my mentor, Timi

9. Amazing places, amazing people