1. PHARMACOKINETICS Allie punke apunke@uthsc.edu
INTRAVASCULAR DOSING
PHARMACOKINETICS
Allie punke

2. CONTINUOUS IV INFUSION
Continuous IV infusion (CIVI) releases the drug at a constant rate.
In contrast to IV Bolus, where the maximum concentration occurs immediately/initial concentration, the maximum concentration for continuous IV infusion occurs at the end of infusion.
Basic equation:
IV Bolus equation: Ct=Co*e^-kt
CIVI equation: Ro/CL*(1-e^-ktinf)(e^-ktpi)

3. Continuous IV infusion
A drug is being given by continuous IV infusion at a rate of 10 µg/hour to reach the steady state concentration of 46 mg/L. What should the infusion rate be increased to in order to reach a steady state concentration of 120 mg/L? 26
10 ug/hour x
46 mg/L mg/L

4. Concentration Time Profiles
Infusion is stopped
Ln concentration
During infusion, at steady state
During infusion, before steady state
Post infusion
Time
General Expression for plasma concentration:
Ct= R0 * (1-e-k * Tinf) (e-k * ( t - Tinf)) CL
Only important before steady state has been achieved
Only important after the infusion is ended

5. During infusion, before steady state
Infusion is stopped
Reasoning:
Since time and time of infusion are equal, t = Tinf, this term becomes e0 = 1.
Ln concentration
During infusion, before steady state
Time
General Expression for plasma concentration:
Ct= R0 * (1-e-k * Tinf) (e-k * ( t - Tinf)) CL
Only important before steady state has been achieved
Only important after the infusion is ended

6. During infusion, at steady state
Reasoning:
Since time and time of infusion are equal, t = Tinf, this term becomes e0 = 1.
When Tinf > Tss = 5*T1/2, then we assume e→∞ = 0. [1 ─ 0 = 1] making this term 1.
Infusion is stopped
Ln concentration
During infusion, at steady state
Time
General Expression for plasma concentration:
Ct= R0 * (1-e-k * Tinf) (e-k * ( t - Tinf)) CL
Only important before steady state has been achieved
Only important after the infusion is ended

7. After infusion, before steady state
Infusion is stopped
Reasoning:
Both terms are necessary for finding Ct.
Ln concentration
After infusion, before steady state
Time
General Expression for plasma concentration:
Ct= R0 * (1-e-k * Tinf) (e-k * ( t - Tinf)) CL
Only important before steady state has been achieved
Only important after the infusion is ended

8. After infusion that ended at steady state
Infusion is stopped
Reasoning:
When Tinf > Tss = 5*T1/2, then we assume e→∞ = 0. [1 ─ 0 = 1] making this term 1.
Ln concentration
Post infusion, after steady state was reached
Time
General Expression for plasma concentration:
Ct= R0 * (1-e-k * Tinf) (e-k * ( t - Tinf)) CL
Only important before steady state has been achieved
Only important after the infusion is ended

9. Continuous IV infusion
A patient is receiving a drug by CIVI at a rate of 800 mg/h to reach a steady state concentration level of 60 mg/L. A blood level drawn at 7 hours results in a concentration of 40 mg/L.
What is the half-life?
Ct=Css (1-e^-ktinf)
40 mg/L=60 mg/L (1-e^-k*7)
K=0.158, Half-life: 4.4 hours
What is the CL?
Css=Ro/CL
60 mg/L=800 mg/h/CL
CL=13.3 L/h
How long to reach steady state?
22 hours

10. Continuous IV Infusion
Maximum concentration after 40 hours?
Cmax=60 mg/L(1-e^-0.158*40)
Cmax=59.89
Suppose the team decides that a level of 47 mg/L is desired. What should the new rate be? 627 mg/h

11. Continuous IV Infusion
A patient is started on a CIVI at a rate of 150 mg/h to reach Css of 6 mg/L. A blood level of 4 mg/L is drawn at 16 hours.
What is the half-life of the drug?
10 hours, k=0.068
4 mg/L=6 mg/L (1-e^-k*16)
What is the CL?
Css=Ro/CL
CL=25L/h
When is steady state reached?
50 hours
What is the maximum concentration achieved if the infusion runs for 36 hours?
Ct=6 mg/L(1-e^-0.068*36 hours)=5.5 mg/L

12. Loading Dose
What LD would we give this pt initially to reach a Css of 6mg/L?
LD=V*Ctarget=6 mg/L*367L=2200 mg
CL=k*V
25L/h=0.068h^-1*V
What LD would we give if the patient has a concentration of 6 mg/L, and we want to achieve a concentration of 14 mg/L?
LD=(Ctarget-Ccurrent)*Vd
LD=(14-6)*367L=2,936 mg

13. Maintenance Dose What would the MD be to achieve 10 mg/L?
MD=CL*Ctarget
MD=25L/h*10 mg/L=250 mg/h

14. Loading and Maintenance Dosing
If CL is increased, this affects:
A. Loading dose
B. Maintenance dose
C. Both
D. Neither loading or maintenance dose

15. CIVI is Stopped
After CIVI is stopped, at hours 2 and 12, the concentrations were 12.9 mg/L and 6 mg/L.
What is the concentration at the end of the infusion?
K=ln(12.9/6)/(12-2)=0.077
Ct=Co*e^-0.077t
C0=15 mg/L
What will the concentration be 24 hours after the infusion was stopped?
Ct=15 mg/L(e^-0.077*24 hours)
Ct= 2.4 mg/L

16. Short term infusion
HP is given a 1000mg dose of vancomycin over a 30 min infusion. Levels are taken at 1 hour and 3 hours and found to be 20mg/L and 14mg/L respectively. The population Vd for vancomycin is 44L.
What is the concentration at 5 hours?
K=0.178
CL=7.85 L/h
DR=2000 mg/h
Ct=255 mg/L (1-e^-0.178*0.5)(e^ *4.5)=9.8mg/L

17. Short term infusion When will the concentration be below 7mg/L?
7mg/L=255 mg/L(1-e^-0.178*0.5)(e^-0.178*tpi)
6.36 hours +0.5 hours=6.88 hours

18. Cumulative Drug in Urine
If Drug X has an fe=1 and a pt is given a dose of 2 grams IVB what will the ΣDu be at t=∞? 2 grams
What if fe=0.75?
Fe*dose=0.75*2 grams=1.5 grams

19. Calculate kr
Calculate kr using the following time points (specifically using Time 4 and 6): kr=ln(139/115.5)/(5-3)=0.092
Time
Du (mg)
Du/t
Rate t*
400 1
356 2
301 4
278
278/2 hours
139 3 6
231
115.5 5 8
199

20. Calculate FE
What is FE if kr is the value found in the previous problem and you have the following information:
A patient is given 400 mg IVB. At 4 hours, the concentration was 16 mg/L and at 10 hours, the concentration was 5 mg/L.
Fe=kr/k
Fe=0.092/0.193=0.47

21. Extravascular Dosing
You have to take into account bioavailability now!
Calculate the bioavailability (F):
72% is not absorbed, 23% does not get first-pass metabolized by the liver, and 80% is metabolized by the gut
F=0.28*0.23*0.2=0.0128

22. Extravascular Dosing
A patient is taking a new drug (500 mg) that has the population values of:
Half-life: 8 hours, Vd: 50 L, kr: h-1, Ka: , 90% reaches the bloodstream
Solve for:
K: h-1
FE:kr/k=0.242
CL (total):4.35L/h

23. Extravascular Dosing (Continued)
CL (renal): kr*Vd, Cltotal*fe=1.05L/h
Cmax: 7.266
Tmax: ln(1.45/0.087)/( )=2 hours
AUC: FD/CL (A/k-A/ka)=0.9*500 mg/(4.35)=103.9

24. Extravascular Dosing
What is it called when the absorption phase is the rate limiting step?
If you were given time points with concentrations and told to assume the drug followed flip-flop kinetics, how could you calculate ka?
Ka=ln(C2/C1)/(Change in time)

25. Summary Things to think about when making calculations for the exam:
1. Is it IVB, CIVI, or oral medications?
2. If it is CIVI, has it been stopped or is it still running?
Double check your work!
Think about if your answers actually make sense based on the information given.
Good luck!