1. SPECTROPHOTOMETER UV 2080 PLUS DOUBLE BEAM

5. INTRODUCTION
UV 2080 Plus is a double beam, general purpose instrument designed to meet the needs of the Conventional Laboratory, This instrument is ideal for various applications, such as: Chemistry, Biochemistry, pharmaceutical, petrochemical, Environmental Protection, Food and Beverage Labs, Water and Waste Water Labs, clinical labs and other fields of quality control and research.
This instrument incorporates a 320×240 dot matrix LCD display for photometric results, easy operation and wavelength range of 190nm to 1100nm. This instrument is ideal for measurements in the visible and ultraviolet wavelength region of the electromagnetic spectrum.
It is suitable for routine applications like Quantitative analysis, kinetics, wavelength scan, multiple components and DNA/ Protein.
PC windows application software make these instrument versatile.
Lamps can turned on/off individually. And also easy to change Pri- aligned Lamps. .

6. Working Principle The spectrophotometer consists of five parts:
Halogen, deuterium lamp (2000h) (Opt: Xenon flash lamp) to supply the light
Czerny Turner Monochromator to isolate the wavelength of interest and eliminate the unwanted second order radiation
A sample compartment to accommodate the sample solution
Two detectors to receive the transmitted light and convert it to an electrical signal
A digital display to indicate absorbance or transmittance. The block diagram (Fig (1) below illustrates the relationship between these parts.

7. Figure 1
In your spectrophotometer, light from the lamp is focused on the entrance slit of the monochromator where the collimating mirror directs the beam onto the grating. The grating disperses the light beam to produce the spectrum, a portion of which is focused on the exit slit of the monochromator by a collimating mirror. From here the beam is passed to a sample compartment through one of the filters, which helps to eliminate unwanted second order radiation from the diffraction grating. Upon leaving the sample compartment, the beam is passed to the silicon photodiode detector and causes the detector to produce an electrical signal that is displayed on the digital display.

8. Features Variable slits (bandwidth)
For stand alone model, all software methods are included as built in standard eliminates the need of software.
Online software upgrade via internet helps to keep it updated.
Data download to PC software expands the data storage to unlimited.

9. Technical Specification
Optical system
Double Beam 1200L/mm
Wavelength range nm
Bandwidth
0.5/1/1.5/2/4/5 nm (Variable)
Spectral Bandpass
1.8nm
Wavelength Accuracy
±0.1 nm
Wavelength reproducibility
±0.002 Abs (0.5 Abs)
Photometric range
-4Abs to 4 Abs; Transmittance: 0-200%
Photometric accuracy
±0.002 Abs (0.5 Abs), ±0.004 Abs (1.0 Abs), ±0.006 Abs (2.0 Abs)
Scan speed
HI, medium , low, Max nm/min
Detector
Photodiodes
Dimensions
600(L) X 450(W) X 200(H) mm
Stray Light
0.02%T Nal at 220 nm, NaNO2 at 340 nm
Photometric repeatability
Less than Abs (0.5 Abs)
Wavelength repeatability
±0.02 nm
Spectra Integration Time
0.02 S

10. Descriptions Filter- Internal holmium Oxide Filter
It is used for Automatic Wavelength Calibration.
Cell- Special Cell position of measure Turbid Sample.
Light Source-Philip Halogen, Deuterium Lamp(2000 h)
Detectors-photodiodes
Instrument Operation Temperature- +15 °C to 35 °C
Optics- Quartz coated Optics
Stability- less than Abs at 700 nm
Photometric Range Absorbance—3 to 3 Abs
Transmittance- 0 % to 400%
Baseline Flatness- ± A
Noise Abs RMS Value at 700 nm
Data output-USB
Scan speed-Hi med Slow, Max nm/min

11. The PC Application Software
● Photometric Mode
● Quantitative test (standard curve)
● Wavelength Scan
● Kinetics
● DNA / Protein
● Multi-wavelength
● System Utility

12. Quantitative test (standard curve) :
Use up to 20 standards to establish standard curve. Four methods for fitting a curve:
1. Linear fit
2. Linear through zero
3. Square fit
4. Cubic fit

13. Kinetics (ABS VS. Time) :
The kinetic mode may be used for time course scanning or reaction rate calculations. Abs vs. time graphs is displayed on the screen in real time. Waiting time measurement time and intervals may be entered. Post run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered.

14. DNA / Protein :
Concentration & DNA purity are quickly and easily calculated :
absorbance ratios 260nm / 280 nm with optional subtracted absorbance at 320nm
DNA Concentration : 62.9 * A * A280
Protein Concentration : 1552 * A * A280
Other wavelengths& factors may be entered

15. Wavelength scan :
Automatically record peaks and valleys. The quantity of channels is unlimited. You can simultaneously store as many as desired. Post run manipulation and processing includes :
1. Re-Scaling axes, curve
2. 1st to 4th derivative
3. Smoothing, combination, zooming, overlap
Multi Wavelength:
Up to 20 wavelengths can be selected and multiple samples can be measured. (Auto cell changer is required to run multiple samples automatically.)

16. Valuable Customers There are 1000 happy customers all over India.
List of Customers /Institutes/Companies in South Region
Max Institute Of Pharmaceutical Sciences, Telangana
Maulana Azad National Urdu University, Hyderabad
Wipro Enterprises (P) Limited, Tumkur, Karnataka
Marine & costal survey Division, Mangalore
Government Of India (GSI), Hyderabad
Birla Institute Of Technology And Science, Hyderabad
Indian Institute of Millets Research (ICAR), Telangana
Central Council for Research in Ayurveda Sciences, Vijayawada

17. Registered Office :   ANALYTICAL TECHNOLOGIES LIMITED Analytical House E67 and E68 RAVI PARK  VASNA ROAD VADODARA GUJARAT INDIA Tel :   TEL /FAX : s: Skype: analyticaltechnologiesltd BROWSE OUR WEB SITES, SOCIAL MEDIA FOR TIME TO TIME UPDATES

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