1. Different expression pattern between human and primate by integration of LTR33 element in MOBP gene
Yu-Na Noh1, Jae-Won Huh2, Dae-Soo Kim3, Hong-Seok Ha1, Kung Ahn1, and Heui-Soo Kim1,*
1. Division of Biological Science, College of Natural Sciences, Pusan National University, Busan , Republic of Korea
2. National Primate Research Center (NPRC), KRIBB, Cheongwon , Republic of Korea
3. Korea Bioinformation Center, KRIBB, Daejeon  , Republic of Korea
Abstract
Introduction
We investigated the association of LTR33 element in transcript variant of MOBP gene. LTR33 belonging to the ERV1 family contributes to the change of coding region of MOBP gene by the production of splicing variant. Real-time RT-PCR analysis using different human tissues shows the dominant expression patterns in AD brain, adult whole brain, spinal cord, and testis tissues. Interestingly, comparative analysis of expression pattern using corresponding tissues between human and primate (Rhesus monkey, Marmoset, Squirrel monkey) shows the different expression patterns. To investigate the evolutionary conservation of LTR33 element of the MOBP gene, various primate DNA samples were used for the PCR amplification and sequencing. Their splicing donor site (GT) are completely conserved and the number of tandem repeated element which could be translated are increased during the evolution of hominoid primates. Taken together, integration event of LTR33 element could affect the coding capacity and expression in different tissues by changing the coding
squences during the primate evolution.
[Structure of Retrovirus]
[Retrovirus life cycle] 8%
Materials & Methods
PCR
Gene
Cloning
Bio
Informatics
Primate DNA
MOBP Gene
Real-time
RT-PCR
[Genome component parts]
[HERV Structure]
*HERV: Human Endogenous Retrovirus
Results & Discussion
MOBP
[Figure 1. Alternative transcripts of MOBP gene]
[Figure 3. Expression analysis of alternative transcripts in
various human tissues]
Human
Gorilla
Orangutan
Gibbon
Old World
monkeys
New World
Prosimians
Million years
LTR33
[Figure 2. Multiple alignment of MOBP gene.]
MOBP_LTR33/GAPDH
Amplified
Plasmid DNA
[Figure 4.Comparative analysis of MOBP gene transcripts in primate]
[Figure 5. Real-time RT-PCR analysis of LTR33 in MOBP gene]
References
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Neurocytology,26: