1. Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4 
Haifa H. Jabara, MSca, Donata Vercelli, MDa, Lynda C. Schneider, MDa, Diane P. Williams, PhDb, Frank S. Genbauffe, PhDb, Louis R. Poissonb, Cory A. Waters, PhDb, Raif S. Geha, MD 
Journal of Allergy and Clinical Immunology 
Volume 95, Issue 4, Pages (April 1995)
DOI: /S (95)
Copyright © 1995 Mosby, Inc. Terms and Conditions

2. FIG. 1
SDS-polyacrylamide gel electrophoresis analysis of DAB389IL-4. Protein samples from three sequential purification steps were prepared in Laemmli buffer, with (A) and without (B) reducing agents (1% 2-mercaptoethanol), and electrophoresed through 4% to 20% gradient gel. Protein bands were visualized with Coomassie Blue R250. Lane 1, Affinity-purified protein; lane 2, after size-exclusion chromatography; lane 3, after ion-exchange chromatography. Molecular weight markers (left) are expressed in kilodaltons.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) )
Copyright © 1995 Mosby, Inc. Terms and Conditions

3. FIG. 2
Inhibition of protein synthesis in phytohemagglutinin-activated human PBMC by DAB389IL-4 is receptor specific. Phytohemagglutinin-stimulated PBMC (1 × 106 cells/ml) were cultured with or without DAB389IL-4 in presence or absence of rIL-2 or rIL-4. After 20-hour incubation, cultures were pulsed with [ 3H]-leucine and harvested 2 hours later. Results represent mean ± SD of triplicate determinations. Figure shows one representative experiment of eight.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) )
Copyright © 1995 Mosby, Inc. Terms and Conditions

4. FIG. 3
DAB389IL-4 inhibits B-cell proliferation induced by PMA and ionophore. B cells (1 × 10 6 cells/ml) were incubated with medium or with PMA (20 ng/ml) plus ionomycin (0.5 μmol/L) in presence or absence of DAB389IL-4. Cultures were pulsed with [3H]thymidine after 72 hours, and harvested 16 hours later. Figure shows mean ± SE of two experiments.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) )
Copyright © 1995 Mosby, Inc. Terms and Conditions

5. FIG. 4
Effect of DAB389IL-4 on IgE synthesis depends on time of addition. B cells (1 × 106 cells/ml) were incubated with rIL-4 (50 U/ml) plus anti-CD40 mAb (5 μg/ml) (A), or with IL-4 (50 U/ml) plus hydrocortisone (10−6 mol/L) (B) . DAB389IL-4 (10−8 mol/L) was added at day 0 or at day 7 after initiation of culture. IgE concentrations in culture supernatants were assessed at days 7 and 12 as described in footnote to Table I. Figure shows results of one representative experiment of three.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) )
Copyright © 1995 Mosby, Inc. Terms and Conditions

6. FIG. 4
Effect of DAB389IL-4 on IgE synthesis depends on time of addition. B cells (1 × 106 cells/ml) were incubated with rIL-4 (50 U/ml) plus anti-CD40 mAb (5 μg/ml) (A), or with IL-4 (50 U/ml) plus hydrocortisone (10−6 mol/L) (B) . DAB389IL-4 (10−8 mol/L) was added at day 0 or at day 7 after initiation of culture. IgE concentrations in culture supernatants were assessed at days 7 and 12 as described in footnote to Table I. Figure shows results of one representative experiment of three.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) )
Copyright © 1995 Mosby, Inc. Terms and Conditions

7. FIG. 5
Effect of DAB389IL-4 on PWM-induced IgM synthesis depends on time of addition. PBMC (1 × 106 cells/ml) were incubated with PWM (1 μg/ml). DAB 389IL-4 (10−8 mol/L) was added at day 0 or at day 5 after initiation of the culture. IgM concentrations in culture supernatants were assessed at days 5 and 10. Figure shows results of one representative experiment three.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) )
Copyright © 1995 Mosby, Inc. Terms and Conditions