1. E. coli O157:H7 food born infection
(A presentation to grow awareness among the general public )
My words: E. coli. Image by David Goodsell. Retrieved from:
Re.:
Shyamal Ghosh,
Walden University
May 7, 2011

2. Foodborne disease
Disease caused by consumption of contaminated foods or beverages. 
Microbes, or pathogens contaminate foods causes different foodborne infections. 
Poisonous chemicals, or other harmful substances also cause foodborne diseases if they are present in food. 
Reference :Centers for Disease Control and Prevention .Retrieved from :

3. Outbreak of E. coli O157:H7 Infections
As of March 22, 2011, 14 persons infected with the outbreak strain of E. coli serotype O157:H7 have been reported
Age & sex: 1 to 70 years, with a median age of 13.5 years. 79% are male.
Among known 13 ill persons, hospitalized 3 or 23% , and none have hemolytic uremic syndrome (HUS). No death reported.
Re:
My words : CDC and many states and the U.S. Department of Agriculture’s Food Safety and Inspection Service (USDA/FSIS) worked together to investigate a multistate outbreak of Escherichia coli O157:H7 infections. By using DNA analysis of E. coli O157:H7 bacteria obtained through diagnostic testing to identify cases of illness that may be part of this outbreak. Distribution of out break :from Maryland (3 cases), New Jersey (2 cases), North Carolina (1 case), Ohio (2 cases) and Pennsylvania (6 cases). Reported dates of illness onset range from January 10, to February 15, 2011.
Reference: Centers for Disease Control and Prevention.(2011). Retrieved from :

4. Mode of infection
Escherichia coli O157:H7 infections can spread through
Contaminated food,
Contaminated drinking water,
Contaminated swimming water, and
from toddler to toddler at a day care center 
Reference : Centers for Disease Control and Prevention.(2011): Retrieved from :  
Re:

5. E. coli Contamination
The most common source of E. coli contamination is cattle, spread through contact with contaminated feces, food and plant products.
Certain foods show very dangerous strains such as:
Undercooked and raw hamburgers
Salami
Alfalfa sprouts
Spinach
Lettuce
Milk (unpasteurized).
My words : The most common source of E. coli contamination in this country is cattle, other domestic and wild animals as well. The bacteria is spread through contact with contaminated feces, food and plant products.
Reference : Centers for Disease Control and Prevention .Retrieved from :
Re.:

6. Consumers precaution protect themselves from foodborne illness.
Cook: Cook thoroughly to be sure to kill bacteria . 
Separate : Keep your food separately from raw food
Chill: Refrigerate your food .
Clean: Wash your food before use.
Report: Report suspected foodborne illnesses to your local health department. 
My words : Cook your food (meal, poultry , eggs etc.) thoroughly to make sure to kill all pathogenic organism . To avoid cross- contaminating foods wash hands, utensils, and cutting boards after they have been in contact with raw meat or poultry.  Put cooked meat on a clean platter, rather one that held the raw meat.  Bacteria can grow quickly at room temperature, so refrigerate foods if they are not going to be eaten within next 4 hours.  Wash fresh fruits and vegetables in running tap water to remove visible dirt and grime.  Wash your hands with soap and water before preparing food.  . If a public health official contacts individual to find our more about an illness, cooperation is important. 
References: Centers for Disease Control and Prevention. Retrieved from : Fight BAC!(TM) education campaign 
Re.:Fight BAC!(TM) education campaign 

7. CDC urges health care professionals to:
Establish the causes in a patient's illness and ways to prevent foodborne disease.
Stool cultures and testing for pathogens –eg. E. coli O157:H7 must be considered.
Reporting suspect cases to concern public health officials.
Encourage any one representation may be the important case of an outbreak.
My words : CDC urges public health professional to encourage the patient with foodborne disease may represent a outbreak of disease, establish the cause, encourage to represent a case by any one may represent an outbreak and all foodborne illness may not have gastrointestinal tract symptoms.
Reference : Centers for Disease Control and Prevention(2011). Retrieved from :
Re.:

8. Food can be made safer in the first place
Buy pasteurized milk
(Now juice pasteurization is a recent important step forward that prevents E. coli O157:H7 infections and many other diseases). 
buy pasteurized fruit juices and ciders.
Reference : Centers for Disease Control and Prevention(2011). Retrieved from:
Re.:

9. Sign -Symptom
The illness it causes is often a severe and bloody diarrhea
Painful abdominal cramps, without much fever. 
Hemolytic uremic syndrome (HUS) can occur several weeks after the initial symptoms. 
Reference : Centers for Disease Control and Prevention (2011). Retrieved from :
Re:

10. Diagnosis Infection is suspected if patients has bloody
E. coli O157 are found by culturing stool samples .
Infection is suspected if patients has bloody
diarrhea usually.
Culturing for E. coli O157:H7 is relatively simple
and not expensive.
Bacteria does colorless colony on sorbitol-
MacConkey (SMAC) agar.
My words : Infection is suspected if patients has bloody diarrhea. Also the absence of bloody diarrhea is not uncommon.
References:
American Medical Association .Diagnosis and Management
of Foodborne Illnesses . Retrieved from:
Centers fro Disease Control and Prevention. Retrieved from :

11. HUS-kidney failure due to E. coli O157:H7 infections
Hemolytic uremic syndrome (HUS)- bloody diarrhea and can be severe if not treated promptly.
Most common age group are young children (4-5 years ).
It causes end stage renal failure in rare instances.
HUS: hemolytic anemia, thrombocytopenia, and acute renal failure.
My words :Hemolytic uremic syndrome (HUS)- bloody diarrhea and can be severe even fatal for 5 to10% cases if not treated promptly. Three common conditions of HUS: hemolytic anemia, thrombocytopenia, and acute renal failure.
Retrieved from : www. Announcements & Events, Food Poisoning Info
Announcements & Events, Food Poisoning Info

12. Home treatment for diarrhea or bloody diarrhea caused by E
Home treatment for diarrhea or bloody diarrhea caused by E. coli infection
Do not use nonprescription antibiotic
Take water frequently or a rehydration
drink
Medical care if moderate dehydration
(dry mouth, dark brown urine with
less volume ).
My words : According to a research antibiotic treatment of children with E. coli O157:H7 infection increases the risk of the hemolytic-uremic syndrome (Wong et al ,2000)
But a meta analysis published in JAMA reported a protective effect or no association (Safdar, Said, Gangnon, Maki ,2002).
References :
Safdar, N., Said, A., Gangnon, R.E., Maki, D.G.(2002).Risk of hemolytic uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 enteritis: a meta-analysis.Jaurnal of American Medical Association, 28,288,8,
Wong, C.S., Jelacic, S., Habeeb, R.L., et al. ( 2000). The risk of the hemolytic-uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 infections. New England Journal of Medicine, 342 ,26, 1930–6.
WebMD.(2011). Retrieved from :

13. Hemolytic Uremic Syndrome (HUS) -blood test for thrombotic thrombocytopenic purpura.
My words :To diagnosis Hemolytic Uremic Syndrome (HUS) easiest lab test use to be done to find out Thrombotic Thrombocytopenic Purpura (TTP) caused by shiga-toxin produced by E. coli bacteria . In HUS kidney can shut down and Patient develop anemia .
Reference :
Arsenault , R. J.(2011).Hemolytic Uremic Syndrome (HUS). Announcements & Events, Food Poisoning Info. Retrieved from : www. Announcements & Events, Food Poisoning Info
& Events, Food Poisoning Info

14. E. Coli under microscope
My words : Gram-negative bacterium E. Coli is a facultative anaerobe . Cells are typically rod shape and near about 2 micro miter long and .5 micrometer diameter with a cell wall of .6 to .7cubic micro miter .It can live on a wide variety of substrate .
Reference:
Centers for Disease Control and Prevention (2011). Retrieved from : ;

15. Ginger extract blocked the toxin responsible for diarrhea
Researchers stated in filed in archive Drugs, Vaccines and Therapeutics , Food and Agriculture on October 2, 2007
“Ginger and its derivatives may be effective herbal supplements for the clinical treatment of enterotoxigenic E. coli diarrhea,"
Retrieved from :

16. E coli ,a gram (-ve) rod and facultative anaerobe
According to CDC near about 70,000 people every year affected with this specific type of E. coli in United States.
The most common type of E. coli is E. coli O157:H7. (Annis , 2010).
My words: Gram-negative rod-shaped bacterium E. coli commonly found lower intestine of warm blooded organisms. Most of E. coli is harm less and serotype o157:H7 cause serious food poisoning in humans.
References:
Vogt, R. L, Dippold, L (2005). Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June–July Public Health Reports, 120 2,174–8.Retrieved from:
Annis K. J. (2010). E. coli contamination outbreak in Maryland . Washington DC Injury Attorney Blog.
Reference : Centers for Disease Control and Prevention .
Re.:

17. Fight Bacteria- Prevention is better than cure.
Clean
Separate
Cook
Chill
Throw away
Check
My words : Public health focuses on prevention of disease . Also preventive measures can be at three levels –primary , secondary and tertiary . Prevention at primary level (check, clean, separate, cook, chill, and throe away ) can assure disease and disability from E. coli. (Schneider, 2011).
Reference: Centers for Disease Control and Prevention. Retrieved from:
Re.:

18. Primary audience of this presentation is general public and other stakeholders are :
Food and Drug Administration. ;
Centers for Disease Control and Prevention. html.
National Institute of Health ;
Department of Agriculture .

19. REFERENCES
American Medical Association .(2011).Diagnosis and Management
of Foodborne Illnesses . Retrieved from:
Annis K. J. (2010). E. coli contamination outbreak in Maryland . Washington DC Injury Attorney Blog.
Arsenault , R. J.(2011).Hemolytic Uremic Syndrome (HUS). Announcements & Events, Food Poisoning Info. Retrieved from : www. Announcements & Events, Food Poisoning Info
References :
American Medical Association .(2011).Diagnosis and Management
of Foodborne Illnesses . Retrieved from:
Annis K. J. (2010). E. coli contamination outbreak in Maryland . Washington DC Injury Attorney Blog.
Arsenault , R. J.(2011).Hemolytic Uremic Syndrome (HUS). Announcements & Events, Food Poisoning Info. Retrieved from : www. Announcements & Events, Food Poisoning Info

20. REFERENCES
Centers for Disease Control and Prevention.(2008) . Retrieved from:
Centers for Disease Control and Prevention (2011). Retrieved from :
Goodsell,D.(2007).E.coliImage.Retrievedfrom:
References:
Centers for Disease Control and Prevention.(2008) . Retrieved from:
Centers for Disease Control and Prevention (2011). Retrieved from :
Goodsell, D.(2007).E.c oli. Image. Retrievedfrom:

21. REFERENCES
Safdar, N., Said, A., Gangnon, R.E., Maki, D.G.(2002).Risk of hemolytic uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 enteritis: a meta- analysis. Jaurnal of American Medical Association, 28,288,8,
Schneider, M.J.(2011). Introduction to public health. Sudbury ,MA : Jones and Bartlett publishers.
The Biotech Weblog (2007) . Retrieved from : weblog.com/ /ginger_extract_may_treat_e_coli_diarrhea.php
References:
Safdar, N., Said, A., Gangnon, R.E., Maki, D.G.(2002).Risk of hemolytic uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 enteritis: a meta- analysis. Jaurnal of American Medical Association, 28,288,8,
Schneider, M.J.(2011). Introduction to public health. Sudbury ,MA : Jones and Bartlett publishers.
The Biotech Weblog (2007) . Retrieved from : weblog.com/ /ginger_extract_may_treat_e_coli_diarrhea.php

22. REFERENCES
Vogt, R. L, Dippold, L (2005). Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June–July Public Health Reports, 120 2,174–8.Retrieved from:
WebMD.(2011). Retrieved from :
Wong, C.S., Jelacic, S., Habeeb, R.L., et al. ( 2000). The risk of the hemolytic-uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 infections. New England Journal of Medicine, 342 ,26, 1930–6.
References:
Vogt, R. L, Dippold, L (2005). Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June–July Public Health Reports, 120 2,174–8.Retrieved from:
WebMD.(2011). Retrieved from :
Wong, C.S., Jelacic, S., Habeeb, R.L., et al. ( 2000). The risk of the hemolytic-uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 infections. New England Journal of Medicine, 342 ,26, 1930–6.