1. Title:
Physiological and Proteomic Alterations in Rice (Oryza sativa L.) Seedlings under Hexavalent Chromium Stress
Journal:
Planta
Authors:
Fanrong Zeng · Xiaojian Wu · Boyin Qiu · Feibo Wu · Lixi Jiang · Guoping Zhang*
Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou , China
* Corresponding author. address: (G. Zhang).

2. Control 2 µM Cr6+ 200 µM Cr6+ Xiushui113 DanK5 a b c f e d
14.4
21.5
31.0
45.0
66.2
174.6 Mr
(kDa) pI 3 10
Supplementary Fig. S1 Two-dimensional electrophoresis (2-DE) gel maps of total leaf proteome of rice seedlings in Xiushui113 (A-C) and DanK5 (D-E) grown under control (A and D), 2 µM Cr6+ (B and E) and 200 µM Cr6+ (C and F) treatments. Chromium treatments were initiated when seedlings were 15 d old. Total leaf protein was isolated from the plants subjected to Chromium treatments for 3 days. A sum of 150 µg of protein was loaded on a pH 3 – 10 IpG strip and protein spots were visualized by silver staining. Mr, relative molecular weight; pI, isoelectric point.