1. Activated neutrophils and platelet microaggregates impede blood filterability through microchannels during simulated extracorporeal circulation 
Yukihiro Yoshimura, MD, Yuji Hiramatsu, MD, PhD, Yukio Sato, MD, PhD, Satoshi Homma, MD, PhD, Yoshiharu Enomoto, MD, Yuji Kikuchi, PhD, Yuzuru Sakakibara, MD, PhD 
The Annals of Thoracic Surgery 
Volume 75, Issue 4, Pages (April 2003)
DOI: /S (02)

2. Fig 1
Diagram of the microchannels. The silicon substrate surface has the compartments partitioned by the banks. The contact of the upper surface of the bank to the glass plate could be made watertight with mechanical pressing alone because of the optical flatness of both surfaces. (D = depth, 4.5 μm; W = width, 6.0 μm; L = length, 10 μm; L′ = length of the terrace, 30 μm.)
The Annals of Thoracic Surgery  , DOI: ( /S (02) )

3. Fig 2
Video-microscope pictures before and during recirculation (donor sample and 30 minutes, 60 minutes, and 120 minutes of recirculation). The microchannels are seen in the center of each picture, and the blood flows from right to left. Plugging of microchannels with neutrophils and platelet microaggregates increased as the recirculation time increased.
The Annals of Thoracic Surgery  , DOI: ( /S (02) )

4. Fig 3
Scanning electron microscope picture of the microchannels. Neutrophil plugs (A) and formed platelet aggregates (B) are detectable. (Original magnification ×3300 for A and ×3500 for B). (N = neutrophil; P = platelet; RBC = red blood cell.)
The Annals of Thoracic Surgery  , DOI: ( /S (02) )

5. Fig 4
The change in transit time of whole blood through microchannels before and during recirculation. Data points are standardized as a percentage of the donor value for each time point. Values are the mean ± standard error of the mean. †p = at 30 minutes, p = at 60 minutes, p = at 120 minutes by single-factor analysis of variance with Bonferroni correction compared with the donor value. (SC = standing control.)
The Annals of Thoracic Surgery  , DOI: ( /S (02) )

6. Fig 5
Expression of CD11b on neutrophil surface before and during recirculation. Data points are standardized as a percentage of the donor value for each time point. Values are the mean ± standard error of the mean. *p < 0.001; †p = by single-factor analysis of variance with Bonferroni correction compared with the donor value. (SC = standing control.)
The Annals of Thoracic Surgery  , DOI: ( /S (02) )

7. Fig 6
Expression of L-selectin on neutrophil surface before and during recirculation. Data points are standardized as a percentage of the donor value for each time point. Values are the mean ± standard error of the mean. *p < 0.001; ‡p = by single-factor analysis of variance with Bonferroni correction as compared with the donor value. (SC = standing control.)
The Annals of Thoracic Surgery  , DOI: ( /S (02) )

8. Fig 7
Expression of neutrophil F-actin before and during recirculation. Data points are standardized as a percentage of the donor value for each time point. Values are the mean ± standard error of the mean. ‡p = at 30 minutes, p = at 60 minutes by single-factor analysis of variance with Bonferroni correction as compared with the donor value. (SC = standing control.)
The Annals of Thoracic Surgery  , DOI: ( /S (02) )