1. Key points: summarized from chapter 14
RNA processing

2. Outline: we will focus on mRNA processing
Overview of the Central Dogma
Link DNA to RNA transcription to proteintranslation
What is a codon?
Gene organization
Introns and exons
What really is a gene?
Steps of pre-mRNA processing
Eukaryotes versus prokaryotes

4. Gene Organization: Is DNA and RNA co-linear?

5. 3 steps of mRNA processing occurs in the nucleus of eukaryotes
5’ CAP
Poly-A tail
Splicing

6. The 5’Cap

9. The Poly-A Tail

10. Cleavage by endonuclease

11. Basic steps of poly-Adenylation

12. Splicing Spliceosome Requires RNA + proteins Self splicing Only RNA!
Function as enzymes
Self splicing
Only RNA!
Function as enzymes!

13. Spliceosome
Meet the spliceosome

14. Splicesome: 3 components are required
Note some important aspects of the 5’ and 3’ ends
5’ phosphodiester of G
2’ OH of A
lariat

17. Where does splicing occurs?

18. Self-Splicing mRNA’s

19. Common characteristic of self-splicing mRNA’s

20. Comments on Alternative processing methods
First: What are the methods of mRNA processing discussed so far?
5’ CAP
3’ Poly A Tail
Intron Splicing

21. Alternative processing methods
Alternative splicing
Multiple 3’cleavage sites
RNA editing

24. And alternative splicing example
Thryoid
BRAIN

26. Silencing or interfering RNAs
siRNA
miRNA

28. Onto translation chapter 15