1. Presentation on
Internship Program
MD. MINHAZUR RAHMAN
ID:

2. Acknowledgement Professor A.A.Z. Ahmad
Chairperson, Department of Mathematics and natural Sciences, BRAC University
Professor Naiyyum Choudhury
Chairman, Bangladesh Atomic Energy Regulatory Authority
Dr. Firdausi Qadri
-Director of Centre for Vaccine Sciences, Senior Scientist & Head of Immunology Unit, icddr,b
-Executive Director, ideSHi

3. Institutions Institute for Developing Science and Health Initiatives
Immunology laboratory,
The International Centre for Diarrheal Disease Research

4. Objectives Practical experience. Skill development.
To be familiar with different environment.
To work with the professional. .

5. Laboratory Learning

6. Icddr,b Identification of Vibrio cholerae and ETEC
Identification and confirmation of V. cholerae by
agglutination using Mab
Dark field microscopy
Dark field microscopy

7. Identification of Enterotoxigenic E. coli by PCR Template Preparation
PCR Master Mix
Gel electrophoresis to confirm the
presence of toxin genes (HTS, HTP and LT)
Gel electrophoresis

8. GM1 ELISA Detection of E. coli heat-stable (ST) and heat-labile toxins
ELISA Test

9. LPS and CT ELISPOT Assay
Detection of the frequency of immunoglobulin-secreting B cells.
Quick Dot Blot
Detection of Colonization factor (CF) by immuno dot blot assay
Detection proteins on the bacterial cell wall.
ELISPOT Assay

10. Flow Cytometry Detection of Human B and T cell by Flow Cytometry
Cell counting, and protein engineering
Fluorescent activated cell sorting machine

11. ideSHi TP test TP test using ELISA
Isolation of peripheral blood mononuclear cells PBMCs
Ficoll Gradient Centrifugation Method
RBC Lysis Method

12. Genomic DNA extraction from human blood sample
Blood collection
Blood cell lysis
Isolation of DNA
PCR
PCR master mix
Thermal cycle
Gel electrophoresis

13. Acute Respiratory and Enteric Infection diagnosis
Isolation and identification of microorganisms
Diagnosis protocols (pneumonia, diarrhea, typhoid)
Gram staining and culture techniques.
Uses of different media like blood agar media.

14. Learning … Using modern equipment for different diagnosis purpose
How to work in a professional laboratory.
How to set up a laboratory.
How to work in groups and different people.
how to maintain the rules regulation and the lab environment.

15. Thank you