1. A Submitter's Guide to GenBank®: Using BankIt for Small-Scale Nucleotide Sequence Submissions
Part 1 December 17, 2014
12/17/14

2. Webinar Logistics - 1
Closed captioning: and enter
All content, including a video recording, will be available at
If you are having technical difficulties, please use the question pod to enter your question
12/17/14

3. Webinar Logistics - 2 Introductions
Bonnie Maidak, Presenter & Webinar Co-creator
Majda Valjavec-Gratian, Webinar Co-creator
Both are part of the NCBI general Help Desk team who are trained to assist data submitters
Michael Fetchko, GenBank Staff Member
All can be reached via
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4. Webinar Logistics - 3
Slides are available now at: ftp://ftp.ncbi.nlm.nih.gov/pub/education/public_webinars/2014/Dec17_BankIt/
Some links on the slides are abbreviated:
Example: <ncbi>/genbank/submit/
To get the full link, replace <ncbi>/ by:
The illustrations in this webinar were generated by using the screen images of the NCBI site or by combining and modifying the images obtained from the Office.com Clip Art (Royalty-free photos and illustrations)
12/17/14

5. Webinar Logistics - 4
If you have questions during the webinar, use the question pod
Responses to all questions will be posted online
Today, ‘TEST’ Submissions in BankIt
– Do not click ‘Finish Submission’
12/17/14

6. Webinar Goals After this webinar, you will be able to:
Recognize if your sequence data are applicable for submission by using BankIt
Understand what you have to know about your sequence before you start your submission process:
Sequence source information
Biological function of the sequence
Become familiar with GenBank submission terminology
Understand and use the FASTA sequence format
Understand steps and input forms in BankIt that allow you to complete your sequence submission
12/17/14

7. Outline Introduction Small-scale Submissions to GenBank®
Kinds of sequence data
Submission tools
The BankIt submission process:
Before you start: What do you need to know about your sequences?
Example submissions of two data sets
Other submission considerations
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8. Introduction
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9. Data Submissions to NCBI
Epigenomics
Data
Gene
Expression
Data
Assembled
Nucleotide
Sequences from Large Projects/ Small-Scale studies
Traces
from
Capillary
(Sanger)
Sequencing
Large Projects
Registrations/
Descriptions
Genomic
Variants
Large Projects
Sample
Registrations/
Descriptions
Short Sequence
Reads from
Next Generation
Sequencing (NGS)
Human Clinical
Data
Genetic Tests
Biomolecular data
Manuscripts
from
NIH-funded
authors
Chemicals
At NCBI, we receive, process, archive, and curate various types of data.
12/17/2014

10. Scope of this Webinar Epigenomics Gene Expression Capillary Traces
Assembled Nucleotide
Sequence from Small-Scale
studies
Project
Description
Variation:
small scale/large
scale variants
Sample
Description
Short Reads
Human Clinical
Data
Genetic Tests
Manuscripts
from
NIH-funded
authors
Chemicals
In this seminar we will focus on the assembled nucleotide sequences from small-scale studies that are submitted to GenBank with a web-based tool BankIt.
12/17/14

11. Sequence Data Submissions to GenBank via BankIt Can Include:
Single or multiple sequences of:
Complete (or partial) sequences > 200 nt long
Protein coding genes
Ribosomal RNA genes (16S, 5S, …)
Internal transcribed spacers (ITS)
Microsatellite markers (but NOT sequence tagged sites, STS)
Complete viral or phage genomes
Complete mitochondrial genomes
Complete chloroplast or other plastids genomes
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12. General Submission Info
About GenBank submissions:
   <ncbi>/genbank/submit/
The kinds of data that will not be accepted by GenBank: <ncbi>/books/NBK53707/#gbankquickstart.what_kind_of_data_will_2
Requirements for GenBank Submissions through BankIt (lists sequence types <200 nt length which are accepted): <ncbi>/WebSub/html/requirements.html
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13. Acceptable Technology for Submissions to GenBank
Sequences obtained by traditional (Sanger) sequencing
Next generation sequences (NGS):
NGS sequences must be assembled to be submitted to GenBank (no raw reads!)
The underlying raw reads do not have to be deposited to SRA (Sequence Read Archive db)
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14. What is BankIt and What You Need to Use It
BankIt is a web-based submission tool where all submission steps are completed on the web
You need a computer with an internet connection and a web browser:
Internet Explorer 9 or above
Current versions of Firefox, Chrome, and Safari
See Browser advice at: <ncbi>/guide/browsers/
12/17/14

15. What is BankIt and What You Need to Use It - 2
NCBI account is required to sign in to BankIt. Establish your (free) account at:
Access BankIt and sign in at:
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16. BankIt Tool Alternatives
Alternative submission tools for GenBank submissions are:
Sequin: downloadable software, most often used alternative ( )
16S rRNA Submission Tool: a specialized tool available in the Submission Portal, only for submission of uncultured Bacterial or Archaeal 16S ribosomal RNA sequences ( )
Barcode Submission Tool: a specialized tool for submission of a 5’ region of the mitochondrial COI genes from animals ( )
tbl2asn: a command line software seldom used as an alternative, designed for different data sets
External submission tools often do not work
12/17/14

17. Outline Introduction Small-scale Submissions to GenBank®
Kinds of sequence data
Submission tools
The BankIt submission process:
Before you start: What do you need to know about your sequences?
Example submissions of two data sets
Other submission considerations
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18. “Dear Sir/Madam
I want to submit one of my gene sequence in gene bank. So can you please help me how to submit my gene's sequence in gene bank. I looking forward to you kind response.
With Best Regards,”
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19. Example 1: Single Sequence Data Submission
GCATGAGATTGGAAGCAGCAATATTGCTTTTATTCATTTAACCTATGTACCAAGTCCAGCTGGAATTAATGAGCAAAAATCTAAACCCACTCAACAGAGTGTTAAAACTTTAAATAAAGCAGGCATTTTCCCCGATCTGATTATTGCTAGAAGCTCTCAAGTATTAACAGATCAAATCAGAAAAAAAGTGGCAATGTTTTGTAATGTTGAGAGCACTTCTATTATTGATAATATTGATGTTTCTACTATTTATGAAATTCCTATATCTTTTTACAAACAAGGCGTACATGAGATTTTAAGTTCTAAGTTGAATATCAAGGTTGATCCAAAAATAGAAGAGCTTTCAAAGCTTGCAGGAGTTATAAAATCTAATTTTTTTGCGCCTAAAAAAATTATTAATATTGCCATTTGTGGTAAATATGCTGAACTTGATGATTCTTATGCGTCAATTCGAGAGTCTTTGGTTCATGTTGGAGCTAATTTGGACTTACTTATTAAAAGCACTCTAATTGATTCTAATGATTTAAATGAGAGTTATTTAAAAGAGTTTGACGGCATTATTGTTCCCGGCGGCTTTGGGGGGAAAGGATATGAGGGTAAAATTATTGCTATTAAATATGCTCGTGAAAATAACATTCCCTTTCT
12/17/14

20. How Would You Describe Your Sequence?
What gene(s) does it represent?
Did you sequence the whole gene or just a part?
Where did you get your sequence from: what organism did you sequence?
Where, when, and how did you obtain your organism?
Is it a genomic sequence or obtained from mRNA?
Is it from the genome or a plasmid, plastid, or mitochondrion?
12/17/14

21. Sequence Source Information
-Where did you get your sequence from: what organism did you sequence?
-Where, when, and how did you obtain your organism?
He wants to know about your sequence source information. Most importantly, what organism is it coming from?
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22. Sequence Source information
-The sequence is from the Borrelia garinii bacterium which causes lyme disease.
-This particular Borrelia garinii is strain Fuji that I bought from a commercial culture collection.
-The Fuji strain was originally isolated from a tick.
12/17/14

23. Molecule Type and Location
Is it a genomic sequence or obtained from mRNA?
Is it from the genome or a plasmid, plastid, or mitochondrion?
She wants to know about your molecule type and the location of the sequence.
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24. Sequence Source information
The sequence is genomic DNA, not from a plasmid or plastid.
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25. Biological Meaning of the Sequence
-What gene(s) does it represent?
-Did you sequence the whole gene or just a part?
He wants to know about the biological function of the sequence.
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26. Sequence Source Information
-I sequenced the CTP synthase (pyrG) gene. It is a protein coding gene. As is typical in bacteria, all of the gene is the coding region, so this is an intronless gene.
-However, I did not sequence the entire gene. Just the middle part.
12/17/14

27. Single Sequence Data Submission
GCATGAGATTGGAAGCAGCAATATTGCTTTTATTCATTTAACCTATGTACCAAGTCCAGCTGGAATTAATGAGCAAAAATCTAAACCCACTCAACAGAGTGTTAAAACTTTAAATAAAGCAGGCATTTTCCCCGATCTGATTATTGCTAGAAGCTCTCAAGTATTAACAGATCAAATCAGAAAAAAAGTGGCAATGTTTTGTAATGTTGAGAGCACTTCTATTATTGATAATATTGATGTTTCTACTATTTATGAAATTCCTATATCTTTTTACAAACAAGGCGTACATGAGATTTTAAGTTCTAAGTTGAATATCAAGGTTGATCCAAAAATAGAAGAGCTTTCAAAGCTTGCAGGAGTTATAAAATCTAATTTTTTTG…
Partial genomic sequence of the CTP synthase (pyrG) gene from the bacterium Borrelia garinii Fuji strain.
The bacterium was isolated from a tick.
We have one more step to do before we are ready to start our BankIt submission:
12/17/14

28. FASTA Format for Nucleotide Sequence Data
In its simplest form:
Begins with the “greater than” sign: “>”
“>” allows software to detect the beginning of a new sequence
Followed by a sequence identifier (‘seqID’)
Temporary, will be replaced when submission is completely processed
If seqID has meaning, this should be repeated in a data field
Keep seqIDs as simple as possible
Both together form a FASTA Definition line: >seq1
The FASTA Definition line is followed by the raw sequence data
12/17/14

29. Additional SeqID Formatting
Create short yet unique Sequence IDs (SeqID or local_ID) composed of letters and/or digits
>Seq1
>Camellia_sinensis_TrEC_Canada_134293_marsh_soil
Spaces not allowed within the SeqID
Use hyphens or underscores to connect sections of the SeqID
Okay
NOT okay (too long)
12/17/14

30. Single Sequence Data Submission
GCATGAGATTGGAAGCAGCAATATTGCTTTTATTCATTTAACCTATGTACCAAGTCCAGCTGGAATTAATGAGCAAAAATCTAAACCCACTCAACAGAGTGTTAAAACTTTAAATAAAGCAGGCATTTTCCCCGATCTGATTATTGCTAGAAGCTCTCAAGTATTAACAGATCAAATCAGAAAAAAAGTGGCAATGTTTTGTAATGTTGAGAGCACTTCTATTATTGATAATATTGATGTTTCTACTATTTATGAAATTCCTATATCTTTTTACAAACAAGGCGTACATGAGATTTTAAGTTCTAAGTTGAATATCAAGGTTGATCCAAAAATAGAAGAGCTTTCAAAGCTTGCAGGAGTTATAAAATCTAATTTTTTTG…
FASTA-formatted sequence ready for our BankIt submission
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31. BankIt Access from NCBI Homepage
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32. BankIt Access from NCBI Homepage - 2
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33.
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34. Login Page
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35. Live Demonstration of BankIt Web Input Forms with a Single Sequence
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36. BankIt Submission Confirmation Page
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37. Example 1: Summary
FASTA formatted nucleotide sequence data important to use
A single sequence can be submitted and annotated using BankIt web input forms
The Source Modifiers step allows for providing sequence source information
Select those modifiers that are pertinent to your sequence
The Features page allows for annotating gene, coding regions and/or other features on the sequence
12/17/14

38. Example 2: Multiple Sequence Submission
GCATGAGATTGGAAGCAGCAATATTGCTTTTATTCATTTAACCTATGTACCAAGTCCAGCTGGAATTAATGAGCAAAAATCTAAACCCACTCAACAGAGTGTTAAAACTTTAAATAAAGCAGGCATTTTCCCCGATCTGATTATTGCTAGAAGCTCTCAAGTATTAACAGATCAAATCAGAAAAAAAGTGGCAATGTTTTGTAATGTTGAGAGCACTTCTATTATTGATAATATTGATGTTTCTACTATTTATGAAATTCCTATATCTTTTTACAAACAAGGCGTACATGAGATTTTAAGTTCTAAGTTGAATATCAAGGTTGATCCAAAAATAGAAGAGCTTTCAAAGCTTGCAGGAGTTATAAAATCTAATTTTTTTGCGCCTAAAAAAATTATTAATATTGCCATTTGTGGTAAATATGCTGAACTTGATGATTCTTATGCGTCAATTCGAGAGTCTTTGGTTCATGTTGGAGCTAATTTGGACTTACTTATTAAAAGCACTCTAATTGATTCTAATGATTTAAATGAGAGTTATTTAAAAGAGTTTGACGGCATTATTGTTCCCGGCGGCTTTGGGGGGAAAGGATATGAGGGTAAAATTATTGCTATTAAATATGCTCGTGAAAATAACATTCCCTTTCT
TGGTGGAACCGTAGGAGATATGGAAAATATTTTATTTATTGAGGCGGTAAGACAAATAAGACAGGAGATTGGGAGTGGTAATATTTCTTTTATTCATTTAACATATGTGCCAAGTCCAGCTGGAATTAATGAGCAAAAATCTAAACCTACTCAACAAAGTGTTAAAACCTTAAATAAAGCAGGTATTTTCCCCGATTTAATTATTGCTAGAAGTTCACAAGTATTGACAGACCAAATCAGAAAAAAAGTGGCAATGTTTTGCAATGTTGAGAGCACTTCTATTATTGACAATGTTGATGTTTCTACTATTTATGAAATTCCTATATCTTTTTATAAGCAGGGTGTACATGAGATTTTAAGCTCTAAGTTAAATATTAAGGTTGATCCAAAAATAGAAGAGCTTTCAAAGCTTGTAGGAGTTATAAAATCTAATTTTTTTGTGCCTAAAAAAATTATTAATATTGCTATTTGTGGTAAATATGCTGAACTTGATGATTCTTATGCATCAATTAGAGAGTCTTTGGTTCATGTTGCAGCCCATTTGGATTTGCTTATTAAAAGCACTTTAATTGATTCTAATGATTTAAATGAGAGCTGTTTAAAAGAGTTTGACGGYATTATTGTTCCTGGCGGCTTTGGAGGCAAAGGATATGAAGGTAAAATTATGGCTATTAAATATGCTCGTGAGAATAATATTCCCTTTCT
TATGGAAAATATTTTATTTATTGAGACAGTAAGGCAAATAAGACATGAGGTTGGAAGTGGTAATATTGCTTTTATTCATTTAACCTATGTACCAAGTCCAGTCGGAATTAATGAGCAAAAATCTAAACCCACTCAACAAAGTGTTAAAACTTTAAATAAAGCAGGCATTTTCCCTGATTTGATTATTGCTAGAAGTTCTCAAGTATTGACAGATCAAATCAGAAAAAAAATAGCAATGTTTTGTAATGTTGAGAGTACTTCTATTATTGATAATATTGATGTTTCTACTATTTATGAAATTCCCATATCTTTTTACAAACAAGGTGTGCATGAGATTTTAAGTTCTAAGTTGAATATTAAGGTTGACCCAAAAATAGAAGGGCTTTCAAGGCTTGTAGGGATTATAAAATCTAATTTTTTTGTGCCTAAAAAAATTATTAATATTGCTATTTGTGGTAAGTATGCTGAACTTGATGATTCTTATGCGTCAATTAGAGAGTCTTTGGTTCATGTTGCAGCTAATTTGGATTTACTTATTAAAAGCACTCTCATTGATTCTAATGATTTAAATGAAAGCTGTTTGAAGGATTTTGACGGTATTATTGTTCCCGGCGGCTTTGGGGGTAAAGGATATGAGGGCAAGATTATTGCTATTAAATATGCTCGTGAGAATAATATTCCCTTTCT
Sequence 1
Sequence 2
Sequence 3
You have three sequences to submit …
12/17/14

39. How Would You Describe Your Sequence?
What gene(s) does it represent?
Did you sequence the whole gene or just a part?
Where did you get your sequence from: what organism did you sequence?
Where, when, and how did you obtain your organism?
Is it a genomic sequence or obtained from mRNA?
Is it from the genome or a plasmid, plastid, or mitochondrion?
12/17/14

40. Description of the Three Sequences
FEATURE ANNOTATION
(gene, CDS)
ORGANISM and
SOURCE MODIFIERS
(strain, isolation source)
Molecule type?
Location?
These sequences are from three different species of Borellia: one obtained from a tick, two from human.
They are genomic DNA sequences; all three represent a partial pyrG gene sequence.
-Borrelia garinii
-Strain=Fuji
-isolation_source= tick.
CTP synthase
(pyrG) gene
Partial CDS
Genomic DNA
-Borrelia burgdorferi
-Strain=297
-Isolation source= human cerebrospinal fluid.
CTP synthase
(pyrG) gene
Partial CDS
Genomic DNA
CTP synthase
(pyrG) gene
Partial CDS
-Borrelia afzelii
-Strain=BO23
-Isolation source= human skin
Genomic DNA
12/17/14

41. Multiple Sequence Submission
GCATGAGATTGGAAGCAGCAATATTGCTTTTATTCATTTAACCTATGTACCAAGTCCAGCTGGAATTAATGAGCAAAAATCTAAACCCACTCAACAGAGTGTTAAAACTTTAAATAAAGC…
>Seq2
TGGTGGAACCGTAGGAGATATGGAAAATATTTTATTTATTGAGGCGGTAAGACAAATAAGACAGGAGATTGGGAGTGGTAATATTTCTTTTATTCATTTAACATATGTGCCAAGT…
>Seq3
TATGGAAAATATTTTATTTATTGAGACAGTAAGGCAAATAAGACATGAGGTTGGAAGTGGTAATATTGCTTTTATTCATTTAACCTATGTACCAAGTCCAGTCGGAATTAATGA…
Sequence 1:
Borrelia garinii
Strain=Fuji
Isolation source=tick
Genomic DNA, partial pyrG
Sequence 2:
Borrelia burgdorferi
Strain=297
Isolation source=human cerebrospinal fluid
Genomic DNA, partial pyrG
Sequence 3:
Borrelia afzelii
Strain=BO23
Isolation source=human skin
Genomic DNA, partial pyrG
12/17/14

42. More FASTA Definition Line Formatting - 1
Enter a single space after each SeqID, and then enter the organism in square brackets, [organism=xxx]
One or more Source Modifiers can be added within square brackets, for example [clone=6b]
End the Definition line with free text of gene and other feature description
12/17/14

43. More FASTA Definition Line Formatting - 2
Information outside the field delimiting characters ‘[]’ (‘[organism=Carpodacus mexicanus]’ for instance), is not parsed by the submission tools into the applicable backend database fields.
The final Definition line is created when GenBank Staff does the final review and processing of the record, just before adding it to the public sequence database.
FASTA formatted files are always in plain txt (*.txt) format, not MS-Word or other format.
12/17/14

44. FASTA Format for Nucleotide Sequence Data
More information about the Definition line is here:
<ncbi>/WebSub/html/help/fasta.html
<ncbi>/Sequin/QuickGuide/sequin.htm#DefinitionLine
12/17/14

45. New Definition Lines
Sequence 1:
Borrelia garinii
Strain=Fuji
Isolation source=tick
partial pyrG CDS
>Seq1 [organism=Borrelia garinii] [strain=Fuji] [isolation_source=tick] CTP synthase (pyrG) gene, partial coding sequence
>Seq2 [organism=Borrelia burgdorferi] [strain=297] [isolation_source=human cerebrospinal fluid] synthase (pyrG) gene, partial coding sequence
>Seq3 [organism=Borrelia afzelii] [strain=BO23] [isolation_source=human skin] CTP synthase (pyrG) gene, partial coding sequence
Sequence 2:
Borrelia burgdorferi
Strain=297
Isolation source=human cerebrospinal fluid
partial pyrG CDS
Sequence 3:
Borrelia afzelii
Strain=BO23
Isolation source=human skin
partial pyrG CDS
Sequence information is now formatted into FASTA definition lines.
Let’s add them to the sequences…
12/17/14

46. FASTA Formatted Data
>Seq1 [organism=Borrelia garinii] [strain=Fuji] [isolation_source=tick] CTP synthase (pyrG) gene, partial coding sequence
GCATGAGATTGGAAGCAGCAATATTGCTTTTATTCATTTAACCTATGTACCAAGTCCAGCTGGAATTAATGAGCAAAAATCTAAACCCACTCAACAGAGTGTTAAAACTTTAAATAAAGCAGGCATTTTCCCCGATCTGATTATTGCTAGAAG…
>Seq2 [organism=Borrelia burgdorferi] [strain=297] [isolation_source=human cerebrospinal fluid] synthase (pyrG) gene, partial coding sequence
TGGTGGAACCGTAGGAGATATGGAAAATATTTTATTTATTGAGGCGGTAAGACAAATAAGACAGGAGATTGGGAGTGGTAATATTTCTTTTATTCATTTAACATATGTGCCAAGTCCAGCTGGAATTAATGAGCAAAAATCTAAACCTAC…
>Seq3 [organism=Borrelia afzelii] [strain=BO23] [isolation_source=human skin] CTP synthase (pyrG) gene, partial coding sequence
TATGGAAAATATTTTATTTATTGAGACAGTAAGGCAAATAAGACATGAGGTTGGAAGTGGTAATATTGCTTTTATTCATTTAACCTATGTACCAAGTCCAGTCGGAATTAATGAGCAAAAATCTAAACCCACTCAACAA…
12/17/14

47. Live Demonstration of BankIt Web Input Forms with Multiple FASTA-formatted Sequences
12/17/14

48. Example 2: Summary
Several sequences can be submitted and annotated using BankIt web input forms when they require simple annotation. For example, a single (same) gene for all sequences.
The Source Modifiers can be included in the FASTA Definition line. For multiple sequences, using the FASTA Definition line makes adding Source Modifier information easier.
5’ partial coding sequences (CDS) can be easily annotated with the web input forms if all translate in the same reading frame (have the same codon_start).
12/17/14

49. Preparing Input Files
A plain text (.txt) file containing sequences in the FASTA format
A Source Modifier file can also be prepared and imported into BankIt
A Feature Table file containing features for each sequence
Use the same SeqIDs in the FASTA file and in the other auxiliary data input files
12/17/14

50. More BankIt Submission Info – Unknown Bases
Use the letter ‘N‘ to indicate an unknown base or a series of Ns to indicate a few unknown bases:
(…TGGTNGGAANNCCCATTGCCAAT…)
Do not add Ns to extend your sequence at the ends:
(…TGGTNGGAANNCCCATTGCCAATNNNNNN)
BankIt does not accept '-' characters in the FASTA sequence
12/17/14

51. More BankIt Submission Info – Sequencing Gaps
Use a string of ‘N’s to indicate sequencing gaps
(…TGGTGGAANNNNNNNNNNNNNNNNCCCA…)
insert the exact number of Ns when gap length is known
insert 100 Ns when the gap length is unknown
BankIt will accept submissions where 'n' is used, as long as the number of 'n's in the entire sequence is less than 50% of the total
Each gap needs to be identified with a miscellaneous feature (‘misc_feature’)
12/17/14

52. More BankIt Submission Info – Sequencing Gaps - 2
The Gapped Sequences Format allows you to define the gap in the FASTA file:
>Seq1
TGGTGGAA
>?unk12
CCCA
>?unk100
CCTGTGTGT
Examples in:
<ncbi>/Sequin/QuickGuide/sequin.htm#GappedSequences
<ncbi>/Sequin/sequin.hlp.html#FASTAFormatforGappedSe quence
12/17/14

53. More BankIt Submission Info – Alignments
BankIt does NOT accept alignment formats
Use Sequin for alignment formats such as:
FASTA+GAP
PHYLIP
NEXUS
12/17/14

54. Additional BankIt Submission Info
If you have questions while creating the BankIt submission, do not click on the ‘Finish Submission’ button on the last ‘Review and Correct’ page.
Send a message to with your question and the BankIt submission number.
Once the ‘Finish Submission’ button is clicked and the submission is in the 'Completed' state, it cannot be edited. A new BankIt submission must be done.
12/17/14

55. For More Information Webinars:
<ncbi>/education/webinars/
General NCBI updates (will include submission/tools updates):
<ncbi>/news/announcements-list/
NCBI News:
<ncbi>/news/
NCBI Insights Blog:
12/17/14

56. Outline Introduction Small-scale Submissions to GenBank®
Kinds of sequence data
Submission tools
The BankIt submission process:
Before you start: What do you need to know about your sequences?
Example submissions of two data sets
Other submission considerations
12/17/14

57. Webinar Goals After this webinar, you will be able to:
Recognize if your sequence data are applicable for submission by using BankIt
Understand what you have to know about your sequence before you start your submission process:
Sequence source information
Biological function of the sequence
Become familiar with GenBank submission terminology
Understand and use the FASTA sequence format
Understand steps and input forms in BankIt that allow you to complete your sequence submission
12/17/14

58. Next BankIt Webinar Wednesday, January 7, 2015, at 1-2 PM EST
Multiple Features within a Sequence
Feature Table file format
BankIt submission for two data sets
12/17/14

59. Provide feedback to webinars@ncbi
12/17/14

60. Questions?
12/17/14

61. A Submitter's Guide to GenBank®: Using BankIt for Small-Scale Nucleotide Sequence Submissions
Part 1 December 17, 2014
12/17/14