1. 27-29 September 2016 in Sanandaj, Kurdistan, Iran.
10th International Congress of Clinical Microbiology
27-29 September 2016 in Sanandaj, Kurdistan, Iran.
Human Papiloma Virus infection and genotypes among HIV Positive women; a descriptive study
Sherko Nasseri¹, Bahram Nikkhoo², Hamid Reza Monavari³, Mohamad Aziz Rasouli⁴, Samane Roohi¹, Shoaib Advay¹
cellular and molecular research center, faculty of Medicine, Kurdistan University of Medical Sciences, sanandaj, Iran
Department of Pathology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran,Iran
Department of Epidemiology& Biostatistics, Kurdistan University of Medical Sciences, Sanandaj, Iran
Corresponding author :
Introduction
Human Papilloma Virus(HPV), small non-enveloped DNA viruses and one of the most common STD agent, human immunodeficiency virus, a RNA virus that attacks and destroys the infection-fighting CD4 Cells of the Immune System and cause immune suppression(1,2).
Results
In this study mean age(SD) of HIV infected women’s were 35.2(+-7.5), crude prevalence of HPV infected women’s among 100 HIV positives was78 (78%); HPV genotypes 11(16%), 16(13%),45(11%) were the most frequencies genotype(figure2). In addition to between all typed sample, 50% was High risk and 50% was Low risk (figure3),Among age groups, the and years old sequentially with 22(28.2%) and 21(26.9%) have most HPV infected individuals. Among HPV infection and age category, significant relationship were not seen (Pv=0.255).
Material and methods
This Descriptive cross-sectional study was performed in between 100 HIV Positive women’s who attend to Iranian Blood Transfusion Organization. ELISA test was used for HIV Screening and Positives were approved by western blot. For HPV detection Cervical swabs and scrapes obtained and transferred into tubes in all cases and transported at 4°C to the virology laboratory.
For detection of HPV, nested polymerase chain reaction (PCR) analysis was performed using MY09/MY11 as the outer primers (Figure1 C) and GP5+/GP6+ as the inner primers (Figure 1 B). For genotyping The INNO-LiPA HPV Genotyping Extra assay (Innogenetics, Gent, Belgium) utilizes a biotinylated consensus primers (SPF10) to amplify a 65-bp region within the L1 ORF of multiple HPV types and based on reverse line blot hybridization. Data were analyzed with SPSS v20 and STATA v12.
Conclusion
Mirzendehdel et al reported 50% prevalence rate of HPV in drug-addicted HIV Positive Iranian women in 2010(3) ,while the present study reported a relatively higher rate of 78% in HIV Positive women. Progressive sightseeing infection with Human Papiloma Virus among at risk Iranian young women show the necessitate of vaccination against this virus as preventive health strategy, specially among at risk population like HIV Positives that is strong risk factor for HPV infection as reported earlier(4).
Figure 2:
all 78 typed HPV, genotypes 11(16%), 16(13%),45(11%) were the most frequencies genotype .
References:
1.Clifford GM, de Vuyst H, Tenet V, Plummer M, Tully S, Franceschi S. Effect of HIV infection on human papillomavirus types causing invasive cervical cancer in Africa. Journal of acquired immune deficiency syndromes
2.Nasseri S, Monavari SH, Keyvani H, Nikkhoo B, Vahabpour Roudsari R, Khazeni M. The prevalence of Human Papilloma Virus (HPV) infection in the oligospermic and azoospermic men. Medical journal of the Islamic Republic of Iran. 2015;29:272.
3.Mirzendehdel S, Nadji SA, Tabarsi P, Baghaei P, Javanmard P, Sigarroodi A, et al. Prevalence of HPV and HIV among female drug addicts attending a drop-in center in Tehran, Iran. International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics. 2010;108(3):254-5.
4.Dartell M, Rasch V, Munk C, Kahesa C, Mwaiselage J, Iftner T, et al. Risk factors for high-risk human papillomavirus detection among HIV-negative and HIV-positive women from Tanzania. Sexually transmitted diseases. 2013;40(9):
Figure3:
39 sample(50%) was classified into high risk group and 39(50%) typed sample belonged to low risk types.
Figure 1 : This figure show A)DNA Marker 100 bp B)2Step Nested PCR 151bp PCR for 11 positive sample C)PCR Check with Outer Primer 450bp D)Negative Control