1. Cell Energetics 2

2. Out line Cellular respiration Photosynthesis Kreb’s cycle
Electron transport chain
Photosynthesis
Photosystem II
Photosystem I

3. Recall from the last class
What happen and where do energy producing processes take place?

4. What happen to the first cell on Earth regarding O2 respiration?
Answer ?
Fermentation follow by anaerobic respiration and aerobic respiration and photosynethesis
Is there any oxygen on Earth at the beginning?
How microorganism survive?

5. Energy producing cell structure

6. Structure of Mitochondria

8. Membrane based energy production
What is the significance of internal structure of mitochondria for ATP production?
Membrane based energy production

9. http://www. eschooltoday

10. The linkage of electron transport, proton pumping, and ATP synthesis
was called the chemiosmotic hypothesis or chemiosmosis coupling

11. Specifically, where does this proton gradient occur inside mitochondria?

12. Mitochondrial membrane
Which membrane allow and not allow biomolecule to pass through?
Why does the inner membrane of mitochondria fold?
Where do electron-transport chain, protein pump and ATP synthase occur?

13. What happen inside mitochondria?
Convert pyruvate to acetyle Co A
Citric acid cycle or Kreb’s cycle
Produce 2 CO2
No O2 involve in this process
Generate ATP, high energy electrons carried by the activated carrier molecules 3 NADH and 1 FADH2

14. Citric acid cycle (Kreb’s cycle)

15. Where does the Kreb’s cycle take place?
Matrix inside mitochondria

16. Electron transport chains
Over 40 proteins
Embeded in inner mitochondrial membrane
Can be divided into 3 large respiratory enzymes
(1) the NADH dehydrogenase complex
(2) the cytochrome b-c1 complex
(3) the cytochrome oxidase complex

17. Why does this process star from NADH and finish at O2 as H2O?

18. Proton motive force both charge and H+ gradient

19. ATP Synthase
the same enzyme occurs in the mito-chondria of animal cells, the chloroplasts of plants and algae, and in the plasma membrane of bacteria

20. Proton motive force drive the transport of other chemicals into and out of mitochondria

21. How electron move from one to another complex
Carriers move along lipid bilayer

22. The linkage of electron transport, proton pumping, and ATP synthesis
was called the chemiosmotic hypothesis or chemiosmosis coupling

26. Cytochrome family
Cytochrome family: Cytochrome c and cytochrome oxidase complex contain Fe in oxidized form. It is reduced to transfer electron to the better electron accepters (cytochrome oxydase complex or oxygen)
Cytochrome oxidase complex prevent O2- (very reactive) to attack cell by tightly bound molecule of O2 until it pick up 4 electron and release as water

28. Chloroplast

36. Carbon fixation

37. Carbon fixation
Glucose synthesis

40. Recommended Books
Lodish, H., Berk, A., Matsudaira, P., Kaiser, C.A., Krieger, M., Scott, M.P., Zipursky, L., Darnell, J Molecular Cell Biology, 5th ed, W.H. Freeman,
Alberts, B., Bray, D., Hopkin, K., Johnson, A., Lewis, J., Raff, M., Roberts, K. & Walter, P Essential cell biology, 3rd ed, Garland Science, New York.